Roles of interleukin-33 in gingival inflammation

• Project/Area Number: 24791997
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Pathobiological dentistry/Dental radiology
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: OHNO Tatsukuni 東京医科歯科大学, 医歯(薬)学総合研究科, 助教 (80435635)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: IL-33 / 歯周炎 / 歯周病 / サイトカイン

Research Abstract

Objectives: IL-33 is involved in Th2-mediated immune responses. We investigated the roles of IL-33 in the gingival inflammation. Methods: WT and IL-33 KO mice received LPS-injection into the gingiva and gingival inflammatory responses were evaluated. Results/Discussion: LPS-injection into gingiva enhanced IL-33 mRNA expression in WT mice. LPS induced inflammatory cytokine mRNA expression levels (IL-1b and TNFa) in IL-33KO mice were higher than wild-type mice. Assessment of alveolar bone loss after LPS injection showed a greater bone loss in both mice as compared with control PBS-injected groups, but IL-33 KO mice showed a significantly higher bone loss. Our results suggest protective roles of IL-33 in early gingival inflammatory responses and chronic alveolar bone loss.

Research Products

• [Journal Article] Galectin-9 enhances cytokine secretion, but suppresses survival and degranulation, in human mast cell line (2014)
  • Author(s): Kojima R, Ohno T, Iikura M, Niki T, Hirashima M, Iwaya K, Tsuda H, Nonoyama S, Matsuda A, Saito H, Matsumoto K, Nakae S
  • Journal Title: PLoS One Volume: 9(1)
  • Peer Reviewed
• [Journal Article] Role of IL-25 and IL-33 in allergic diseases (2013)
  • Author(s): Morita H, Unno H, Arae K, Ohno T, Saito H, Matsumoto K, Nakae S
  • Journal Title: Arerugi Volume: 62(8) Pages: 924-35
  • NAID: 110009649375
  • Peer Reviewed
• [Journal Article] IL-33-mediated innate response and adaptive immune cells contribute to maximum responses of protease allergen-induced allergic airway inflammation (2013)
  • Author(s): Kamijo S, Takeda H, Tokura T, Suzuki M, Inui K, Hara M, Matsuda H, Matsuda A, Oboki K, Ohno T, Saito H, Nakae S, Sudo K, Suto H, Ichikawa S, Ogawa H, Okumura K, Takai T
  • Journal Title: J Immunol Volume: 190(9) Pages: 4489-99
  • Peer Reviewed
• [Journal Article] Role of interleukin-33 in innate-type immune cells in allergy (2013)
  • Author(s): Nakae S, Morita H, Ohno T, Arae K, Matsumoto K, Saito H
  • Journal Title: Allergol Int Volume: 62(1) Pages: 13-20
  • NAID: 130004477181
  • Peer Reviewed
• [Journal Article] Epithelial cell-derived IL-25, but not Th17 cell-derived IL-17 or IL-17F, is crucial for murine asthma (2012)
  • Author(s): Suzukawa M, Morita H, Nambu A, Arae K, Shimura E, Shibui A, Yamaguchi S, Suzukawa K, Nakanishi W, Oboki K, Kajiwara N, Ohno T, Ishii A, Körner H, Cua DJ, Suto H, Yoshimoto T, Iwakura Y, Yamasoba T, Ohta K, Sudo K, Saito H, Okumura K, Broide DH, Matsumoto K, Nakae S
  • Journal Title: J Immunol Volume: 189(7) Pages: 3641-52
  • Peer Reviewed
• [Journal Article] Interleukin-33 in allergy (2012)
  • Author(s): Ohno T, Morita H, Arae K, Matsumoto K, Nakae S
  • Journal Title: Allergy Volume: 67(10) Pages: 1203-14
  • Peer Reviewed
• [Journal Article] ST2 requires Th2-, but not Th17-, type airway inflammation in epicutaneously antigen- sensitized mice (2012)
  • Author(s): Morita H, Arae K, Ohno T, Kajiwara N, Oboki K, Matsuda A, Suto H, Okumura K, Sudo K, Takahashi T, Matsumoto K, Nakae S
  • Journal Title: Allergol Int Volume: 61(2) Pages: 265-73
  • NAID: 130004477129
  • Peer Reviewed
• [Journal Article] Interleukin-33 in allergy. (2012)
  • Author(s): Ohno T, Morita H, Arae K, Matsumoto K, Nakae S.
  • Journal Title: Allergy. Volume: 67 Issue: 10 Pages: 1203-14
  • DOI: 10.1111/all.12004
  • Peer Reviewed
• [Presentation] Dual Roles of Interleukin-33 in LPS-induced Acute and Chronic Gingival Inflammation (2013)
  • Author(s): Tatsukuni Ohno, Chenyang Zhang, Shogo Maekawa, Miyuki Azuma
  • Organizer: 2nd Meeting of IADR-APR
  • Place of Presentation: Bangkok
• [Presentation] マウスLPS 誘導歯周炎におけるIL-33 の関与 (2012)
  • Author(s): 大野建州, 前川祥吾, 和泉雄一, 東みゆき
  • Organizer: 第54回歯科基礎医学会学術大会・総会
  • Place of Presentation: 郡山
• [Presentation] LPS誘導性歯周病マウスモデルにおけるIL-33の解析 (2012)
  • Author(s): 大野建州、張晨陽、東みゆき
  • Organizer: 第54回歯科基礎医学会
  • Place of Presentation: 郡山
• [Presentation] LPS誘導性歯周病マウスモデルにおけるIL-33の解析 (2012)
  • Author(s): 大野建州、張晨陽、東みゆき
  • Organizer: 第77回口腔病学会
  • Place of Presentation: 東京