Functional analysis of oral cancer stem cell related gene, SPRR1B

Research Project

Project/Area Number	24792005
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Pathobiological dentistry/Dental radiology
Research Institution	Sapporo Medical University
Principal Investigator	MICHIFURI Yoshitaka 札幌医科大学, 医学部, 研究員 (00457722)
Project Period (FY)	2012-04-01 – 2015-03-31
Project Status	Completed (Fiscal Year 2014)
Budget Amount *help	¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000) Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000) Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000) Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords	口腔がん / がん幹細胞 / SPRR1B / 口腔扁平上皮癌
Outline of Final Research Achievements	We isolated oral cancer stem-like cells(CSCs)/cancer-initiating cells(CICs) by aldefluor assay. A cDNA microarray analysis revealed that SPRR1B is preferentially expressed in oral CSCs/CICs. Gene overexpression and gene knockdown experiments revearled that SPRR1B has a role in the cell growth of oral CSCs/CICs by activation of RAS signal. These observations indicate that SPRR1B might be a promising molecular target for treatment resistant oral CSCs/CICs.