Nanoscale analysis of enamel-adhesive interface with using FIB-TEM.

• Project/Area Number: 24792016
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Conservative dentistry
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: TAKAGAKI Tomohiro 東京医科歯科大学, 医歯(薬)学総合研究科, 助教 (60516300)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: ＦＩＢ－ＴＥＭ / 機能性モノマー / FIB-TEM / QCM-D

Outline of Final Research Achievements

An FIB (Focused ion beam) micro-sampling technique has been used for specimen preparation for TEM microscopy of semiconductor devices and other electronic materials as it allows direct and site-specific specimen preparation from a bulky sample. This technique is also useful for hard-tissue specimens since they are often deformed by mechanical forces applied during the initial cutout in the preparation. We have applied this technique for preparation of enamel-adhesive interface specimen for TEM observation. The purpose of this study was to analyze enamel-adhesive interface with using FIB-TEM technique. Micro-sampling method with FIB is practical and reliable method of TEM cross-section specimen preparation of biomaterial-hard tissue interfaces.

Research Products

• [Journal Article] The role of MDP in a bonding resin of a two-step self-etching adhesive system (2015)
  • Author(s): Matsui N, Takagaki T, Sadr A, Ikeda M, Ichinose S, Nikaido T, Tagami J.
  • Journal Title: Dental Materials Journal Volume: 34 Issue: 2 Pages: 227-233
  • DOI: 10.4012/dmj.2014-205
  • NAID: 130005062415
  • ISSN: 0287-4547, 1881-1361
  • Peer Reviewed / Open Access
• [Journal Article] Immobilization of phosphate monomers on collagen induces biomimetic (2015)
  • Author(s): Nurrohman H, Nakashima S, Takagaki T, Sadr A, Nikaido T, Asakawa Y, Uo M, Marshall SJ, Tagami J.
  • Journal Title: Biomed Mater Eng. Volume: 25(1) Issue: 1 Pages: 88-99
  • DOI: 10.3233/bme-141243
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Phosphoric acid-etching promotes bond strength and formation of ABRZ on enamel. (2013)
  • Author(s): Li N, Nikaido T, Sadr A, Takagaki T, Chen J-H, Tagami J.
  • Journal Title: Operative Dentistry Volume: 38(1) Issue: 1 Pages: 82-90
  • DOI: 10.2341/11-422-l
  • Peer Reviewed
• [Journal Article] Development and evaluation of a low-erosive apple juice drink with Phosphoryl-Oligosaccharides of Calcium. (2013)
  • Author(s): Mita H, Kitasako Y, Takagaki T, Sadr A, Tagami J
  • Journal Title: Dent Mater J Volume: 32(2) Pages: 212-8
  • NAID: 10031151403
  • Peer Reviewed
• [Journal Article] Dentin bonding performance and ability of four MMA-based adhesive resins to prevent demineralization along the hybrid layer. (2012)
  • Author(s): Nurrohman H, Nikaido T, Takagaki T, Sadr A, Waidyasekera K, Kitayama S, Ikeda M, Tagami J
  • Journal Title: J Adhes Dent Volume: 14(4) Pages: 339-48
  • Peer Reviewed
• [Journal Article] Apatite crystal protection against acid-attack beneath resin-dentin interface with four adhesives: TEM and crystallography evidence. (2012)
  • Author(s): Nurrohman H, Nikaido T, Takagaki T, Sadr A, Ichinose S, Tagami J
  • Journal Title: Dent Mater Volume: 28(7) Issue: 7 Pages: e89-e98
  • DOI: 10.1016/j.dental.2012.04.025
  • Peer Reviewed
• [Journal Article] Effect of lining with a flowable composite on internal adaptation of direct composite restorations using all-in-one adhesive systems. (2012)
  • Author(s): Yahagi C, Takagaki T, Sadr A, Ikeda M, Nikaido T, Tagami J
  • Journal Title: Dent Mater J Volume: 31(3) Pages: 481-8
  • NAID: 10030315546
  • Peer Reviewed
• [Presentation] Evaluation of Dentin Bonding Performance and ABRZ Formation of Newly-developed Adhesives (2015)
  • Author(s): Guan Rui, Naoko Matsui, Takaaki Sato, Tomohiro Takagaki, Toru Nikaido, Junji Tagami
  • Organizer: IADR General session 2015
  • Place of Presentation: ボストン アメリカ合衆国
  • Year and Date: 2015-03-11 – 2015-03-14
• [Presentation] Morphological and mechanical evaluation of enamel-adhesive interface of self-etch systems (2015)
  • Author(s): Takaaki Sato, Tomohiro Takagaki, Naoko Matsui, Alireza Sadr, Toru Nikaido, Junji Tagami
  • Organizer: IADR General session 2015
  • Place of Presentation: ボストン アメリカ合衆国
  • Year and Date: 2015-03-11 – 2015-03-14
• [Presentation] ジルコニアセラミックス表面処理におけるリン酸系機能性モノマーの役割 (2014)
  • Author(s): 荒井昌海、佐藤隆明、高垣智博、二階堂徹、田上順次
  • Organizer: 第141回日本歯科保存学会秋季学術大会
  • Place of Presentation: 山形テルサ 山形市 山形県
  • Year and Date: 2014-10-30 – 2014-10-31
• [Presentation] SEM observation of acid-base resistant zone in newly-developed bonding system (2014)
  • Author(s): Guan Rui、Naoko Matsui、 Tomohiro Takagaki、Toru Nikaido、Junji Tagami
  • Organizer: 第140回日本歯科保存学会秋季学術大会
  • Place of Presentation: 滋賀県立芸術劇場 大津市 滋賀県
  • Year and Date: 2014-06-19 – 2014-06-20
• [Presentation] コンポジットレジンの耐摩耗性に関する新規評価法の検討 (2014)
  • Author(s): 成瀬 由己、髙垣 智博、松井 七生子、佐藤 隆明、二階堂 徹、池田 正臣、田上 順次
  • Organizer: 第140回日本歯科保存学会秋季学術大会
  • Place of Presentation: 滋賀県立芸術劇場 大津市 滋賀県
  • Year and Date: 2014-06-19 – 2014-06-20
• [Presentation] TEM Observation of Acid-base Resistant Zone on Enamel. (2013)
  • Author(s): Li N, Nikaido T, Takagaki T, Chen JH, Tagami J.
  • Organizer: 5th International Adhesive Dentistry Meeting
  • Place of Presentation: Philadelphia
• [Presentation] QCM-D Analysis of Chemical Adsorption of Functional-monomer with HAp Sensor. (2013)
  • Author(s): Takagaki T, Nikaido T, Matsui N, Sato T, Tagami J.
  • Organizer: 5th International Adhesive Dentistry Meeting
  • Place of Presentation: Philadelphia
• [Presentation] The Role of MDP in Two-step Self-etching Bonding Agent. (2013)
  • Author(s): Matsui N, Takagaki T, Nikaido T, Ichinose S, Ikeda M, Tagami J.
  • Organizer: 5th International Adhesive Dentistry Meeting
  • Place of Presentation: Philadelphia
• [Presentation] SEM observations of enamel acid-base resistant zone using self-etch adhesive systems. (2013)
  • Author(s): Sato T, Takagaki T, Matsui N, Nikaido T, Tagami J.
  • Organizer: Internatioanl Summer Program TMDU
  • Place of Presentation: 東京
• [Presentation] エナメル質と各種ボンディング材との接着界面における ABRZ の評価. (2013)
  • Author(s): 佐藤隆明，高垣智博，松井七生子，二階堂徹，田上順次．
  • Organizer: 第138回日本歯科保存学会春季学術大会
  • Place of Presentation: 福岡
• [Presentation] Ultrastructural assessment of tooth-resin interface using FIB-TEM technique. (2012)
  • Author(s): Baksh TA, Sadr A, Shimada Y, Mandurah M, Takagaki T, Tagami J
  • Organizer: 90th General Session of IADR
  • Place of Presentation: Iguacu falls, Brazil
• [Presentation] Interface Observation of Acid-base Resistant Zone Formed by Adhesive Systems. (2012)
  • Author(s): 15. Di Francescantonio M, Nurrohman H, Takagaki T, Giannini M, Nikaido T, Tagami J
  • Organizer: 90th General Session of IADR
  • Place of Presentation: Iguacu falls, Brazil
• [Presentation] Bond Strength of Resin Cements to Dentin Using Resin Coating. (2012)
  • Author(s): 17. Giannini M, Cantanhede De Sa RB, Mabrosano G, Takagaki T, Nikaido T, Tagami J
  • Organizer: 90th General Session of IADR
  • Place of Presentation: Iguacu falls, Brazil
• [Presentation] Nanoleakage Expression in Hybrid Layer and Acid-base Resistant Zone. (2012)
  • Author(s): Nikaido T, Nurrohman H, Waidyasekera K, Takagaki T, Sadr A, Tagami J
  • Organizer: 90th General Session of IADR
  • Place of Presentation: Iguacu falls, Brazil
• [Presentation] Raman Microscopic Analysis of ABRZ Using Different Functional Monomers. (2012)
  • Author(s): Takagaki T, Nikaido T, Sadr A, Tagami J
  • Organizer: 90th General Session of IADR
  • Place of Presentation: Iguacu falls, Brazil
• [Book] 削るう蝕、削らないう蝕 (2013)
  • Author(s): 二階堂徹、高垣智博、田上順次．
  • Total Pages: 216
  • Publisher: クインテッセンス出版
• [Book] ライフステージと歯内療法．歯の長期保存のために (2013)
  • Author(s): 二階堂徹、高垣智博、田上順次．
  • Total Pages: 164
  • Publisher: デンタルダイヤモンド社