| Project/Area Number |
24792020
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Conservative dentistry
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| Research Institution | Osaka University |
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Principal Investigator |
ASAHI Yoko 大阪大学, 歯学部附属病院, 助教 (50456943)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | バイオフィルム / エピガロカテキンガレート / in situバイオフィルムモデル / 歯肉縁上バイオフィルム / Porphyromonas gingivalis / in situ device |
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| Research Abstract |
The new in situ biofilm model which can form the dental biofilm comprised of multiple bacterial species was made, and chronological changes of the dental biofilm were investigated. The bacterial count showed logarithmic growth till 24 hours after biofilm formation, and showed the gradual increase till 4days. Moreover, the effects of epigallocatechin gallate (EGCg), which has an antibacterial actvity, on oral bacterial biofilm were investigated. Significant decrease of ATP levels of bacteria was seen in EGCg-treated preformed biofilm. The inhibitory effect of EGCg on biofilm formation was also observed. It was suggested that the prevention mechanism of EGCg on biofilm formation differed from preformed biofilm inhibitory mechanism.
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