| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Research Abstract |
To examine each of BO33-, Sr2+, Al3+, Na+, SiO32-, and F-, we succeed to make solutions with same counter ions such as H3BO3, Sr(NO3)2, Al(NO3)3, Na2SiO3, and NaF at the neutral pH. MC3T3-E1 cells were seeded and each solution was added. Cell proliferation was examined by MTT assay. Significant increase in the cell number was obtained by Sr(NO3)2. Culture with H3BO3 for 3 days and NaF demonstrated inhibition of cell proliferation in a dose-dependent manner. MC3T3-E1 cells proliferate well in the presence of Sr(NO3)2, indicating the positive effects of Sr2+ ion on osteoblasts. On the contrary, BO33- and F- appear to have negative influences on osteoblastic proliferation.
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