| Project/Area Number |
24792028
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Conservative dentistry
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
MAEDA Hidefumi 九州大学, 病院, 講師 (10284514)
WADA Naohisa 九州大学, 病院, 講師 (60380466)
AKAMINE Akifumi 九州大学, 歯学研究院, 教授 (00117053)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | interleukin-11 / mechanical stress / periodontal ligament |
|---|
| Research Abstract |
Positive interleukin-11 (IL-11) staining was observed in the periodontal ligament (PDL) of rat maxillae and in cultures of the human primary PDL cells (HPDLCs). In HPDLCs exposed to stretch, gene and protein expression of IL-11 were up-regulated concomitant with an increase in angiotensin II (Ang II) and via AT2, Ang II receptor. Recombinant human IL-11 (rhIL-11) increased mRNA expression of the cementoblast-specific marker, CP-23, and the osteoblastic markers and promoted proliferation in human PDL progenitor cells (cell line 1-17). In addition, rhIL-11 also increased the degree of mineralized nodule formation in cell line 1-17 cultures treated with CaCl2.
Mechanical loading appears to control proliferation and osteo/cementoblastic differentiation of human PDL stem/progenitor cells through IL-11 regulation of Ang II and AT2.
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