| Project/Area Number |
24792035
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Conservative dentistry
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| Research Institution | Tokyo Dental College |
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Principal Investigator |
SATO Masaki 東京歯科大学, 歯学部, 助手 (80598855)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 歯内治療薬 / 象牙芽細胞 / TRPチャネル / カルシウム / 電気生理 / 培養 / 国際情報交換 |
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| Research Abstract |
Eugenol has been used since ancient times as a local anesthetic for toothaches. Despite its long history of use, relatively little is known about its physiological effects. In the present study, we investigated the effects of eugenol on intracellular free Ca2+ concentration ([Ca2+]i) in mouse odontoblasts. Application of eugenol increased [Ca2+]i transiently in a concentration-dependent manner. Removal of extracellular Ca2+ abolished this effect, indicating that eugenol activated Ca2+ entry. Capsazepine, an antagonist of TRPV1 channels, inhibited the increase in [Ca2+]i. Additionally, after a transient increase in [Ca2+]i, continuous administration of eugenol induced sensitizing and subsequent desensitizing effects on Ca2+ entry via TRPV1 channels. Therefore, eugenol modulates the sensitization/desensitization of TRPV1 channel activation in odontoblasts, which may mediate the analgesic effects of eugenol on dentinal sensitivity.
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