Effects and application of endodontics chemicals: Function of between received oxious stimulus and reactive dentin formaing.

• Project/Area Number: 24792035
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Conservative dentistry
• Research Institution: Tokyo Dental College
• Principal Investigator: SATO Masaki 東京歯科大学, 歯学部, 助手 (80598855)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 歯内治療薬 / 象牙芽細胞 / TRPチャネル / カルシウム / 電気生理 / 培養 / 国際情報交換

Research Abstract

Eugenol has been used since ancient times as a local anesthetic for toothaches. Despite its long history of use, relatively little is known about its physiological effects. In the present study, we investigated the effects of eugenol on intracellular free Ca2+ concentration ([Ca2+]i) in mouse odontoblasts. Application of eugenol increased [Ca2+]i transiently in a concentration-dependent manner. Removal of extracellular Ca2+ abolished this effect, indicating that eugenol activated Ca2+ entry. Capsazepine, an antagonist of TRPV1 channels, inhibited the increase in [Ca2+]i. Additionally, after a transient increase in [Ca2+]i, continuous administration of eugenol induced sensitizing and subsequent desensitizing effects on Ca2+ entry via TRPV1 channels. Therefore, eugenol modulates the sensitization/desensitization of TRPV1 channel activation in odontoblasts, which may mediate the analgesic effects of eugenol on dentinal sensitivity.

Research Products

• [Journal Article] Plasma membrane stretch activates transient receptor potential vanilloid and ankyrin channels in Merkel cells from hamster buccal mucosa (2014)
  • Author(s): Soya, M., Sato, M., Sobhan, U., Tsumura, M., Ichinohe, T., Tazaki, M., and Shibukawa, Y
  • Journal Title: Cell Calcium Volume: 55 Pages: 208-218
• [Journal Article] Plasma membrane stretch activates transient receptor potential vanilloid and ankyrin channels in Merkel cells from hamster buccal mucosa (2014)
  • Author(s): Manabu Soya, Masaki Sato, Ubaidus Sobhan, Maki Tsumura, Tatsuya Ichinohe, Masakazu Tazaki, Yoshiyuki Shibukawa
  • Journal Title: Cell Calcium Volume: 印刷中 Issue: 4 Pages: 208-218
  • DOI: 10.1016/j.ceca.2014.02.015
  • Peer Reviewed
• [Journal Article] Functional Expression of TRPM8 and TRPA1 Channels in Rat Odontoblasts (2013)
  • Author(s): Tsumura, M., Sobhan, U., Sato, M., Shimada, M., Nishiyama, A., Kawaguchi, A., Soya, M., Kuroda, H., Tazaki, M., and Shibukawa, Y
  • Journal Title: PloS One Volume: 8
• [Journal Article] Immunolocalization and distribution of functional temperature-sensitive TRP channels in salivary glands (2013)
  • Author(s): Sobhan, U., Sato, M., Shinomiya, T., Okubo, M., Tsumura, M., Muramatsu, T., Kawaguchi, M., Tazaki, M., and Shibukawa, Y
  • Journal Title: Cell Tissue Res Volume: 354 Pages: 507-519
• [Journal Article] Hypotonic-induced stretching of plasma membrane activates transient receptor potential vanilloid channels and sodium-calcium exchangers in mouse odontoblasts (2013)
  • Author(s): Sato, M., Sobhan, U., Tsumura, M., Kuroda, H., Soya, M., Masamura, A., Nishiyama, A., Katakura, A., Ichinohe, T., Tazaki, M., et al
  • Journal Title: J. Endod Volume: 39 Pages: 779-787
• [Journal Article] Sodium-calcium exchangers in rat trigeminal ganglion neurons (2013)
  • Author(s): Kuroda, H., Sobhan, U., Sato, M., Tsumura, M., Ichinohe, T., Tazaki, M., and Shibukawa, Y
  • Journal Title: Mol. Pain Volume: 9 Pages: 22-22
• [Journal Article] 象牙芽細胞におけるtransient receptor potential melastatin subfamily member 8 チャネルの発現と検索 (2013)
  • Author(s): 高橋史子, 津村麻記, Sobhan Ubaidus, 佐藤正樹, 田崎雅和, 澁川義幸
  • Journal Title: 医学と生物 Volume: 157 (6) Pages: 985-990
• [Journal Article] グアヤコールは象牙芽細胞の細胞内Ca^<2+>流入を活性化する (2013)
  • Author(s): 隝田みゆき, 津村麻記, 佐藤正樹, Sobhan Ubaidus, 大多和由美, 山下秀一郎, 田崎雅和, 澁川義幸
  • Journal Title: 歯科学報 Volume: 113 (6) Pages: 597-602
• [Journal Article] Functional expression of TRPM8 and TRPA1 channels in rat odontoblasts. (2013)
  • Author(s): Maki Tsumura, Ubaidus Sobhan, Masaki Sato, Miyuki Shimada, Akihiro Nishiyama, Aya Kawaguchi, Manabu Soya, Hidetaka Kuroda, Masakazu Tazaki, Yoshiyuki Shibukawa
  • Journal Title: PLoS One Volume: 8 Issue: 12 Pages: e82233-e82233
  • DOI: 10.1371/journal.pone.0082233
  • Peer Reviewed
• [Journal Article] Hypotonic-induced stretching of plasma membrane activates TRPV channels and sodium-calcium exchangers in mouse odontoblasts (2013)
  • Author(s): Masaki Sato, Ubaidus Sobhan, Maki Tsumura, Hidetaka Kuroda, Manabu Soya, Aya Masamura, Akihiro Nishiyama, Akira Katakura, Tatsuya Ichinohe, Masakazu Tazaki, Yoshiyuki Shibukawa
  • Journal Title: Journal of endodontics Volume: 未定 Issue: 6 Pages: 779-787
  • DOI: 10.1016/j.joen.2013.01.012
  • Peer Reviewed
• [Journal Article] Sodium-calcium exchangers in rat trigeminal ganglion neurons. (2013)
  • Author(s): Hidetaka Kuroda, Ubaidus Sobhan, Masaki Sato, Maki Tsumura, Tatsuya Ichinohe, Masakazu Tazaki, Yoshiyuki Shibukawa
  • Journal Title: Molecular Pain Volume: 9 Pages: 22-22
  • DOI: 10.1186/1744-8069-9-22
  • Peer Reviewed
• [Journal Article] 象牙芽細胞におけるtransient receptor potential melastatin subfamily member 8 チャネルの発現と検索 (2013)
  • Author(s): 高橋史子、津村麻記、Sobhan Ubaidus、佐藤正樹、田崎雅和、澁川義幸
  • Journal Title: 医学と生物 Volume: 157 Pages: 985-990
  • Peer Reviewed
• [Journal Article] グアヤコールは象牙芽細胞の細胞内Ca2+流入を活性化する (2013)
  • Author(s): 隝田みゆき、津村麻記、佐藤正樹、Sobhan Ubaidus、大多和由美、山下秀一郎、田﨑雅和、澁川義幸
  • Journal Title: 歯科学報 Volume: 113 Pages: 597-602
  • Peer Reviewed
• [Journal Article] Immunolocalization and distribution of functional temperature-sensitive TRP channels in the salivary glands (2013)
  • Author(s): U Sobhan, M Sato, T Shinomiya, M Okubo, M Tsumura, T Muramatsu, M Kawaguchi, M Tazaki, Y Shibukawa
  • Journal Title: Cell and Tissue Research Volume: 354 Issue: 2 Pages: 507-519
  • DOI: 10.1007/s00441-013-1691-x
  • Peer Reviewed
• [Journal Article] TRPV1-mediated calcium signal couples with cannabinoid receptors and sodium-calcium exchangers in rat odontoblasts (2012)
  • Author(s): Tsumura, M., Sobhan, U., Muramatsu, T., Sato, M., Ichikawa, H., Sahara, Y., Tazaki, M., and Shibukawa, Y
  • Journal Title: Cell Calcium Volume: 52 Pages: 124-136
• [Journal Article] Bradykinin up-regulates voltage-dependent sodium channels in human odontoblast (2012)
  • Author(s): Tazaki, M., Ichikawa M., Tsumura M., Sato M., Sobhan U., Endoh T., Shibukawa Y
  • Journal Title: Med. Biol Volume: 156 Pages: 404-409
• [Journal Article] TRPV1-mediated calcium signal couples with cannabinoid receptors and sodium-calcium exchangers in rat odontoblasts (2012)
  • Author(s): M Tsumura, U Sobhan, T Muramatsu, M Sato, H Ichikawa, Y Sahara, M Tazaki, Y Shibukawa
  • Journal Title: Cell Calcium Volume: 52 Issue: 2 Pages: 124-136
  • DOI: 10.1016/j.ceca.2012.05.002
  • Peer Reviewed
• [Presentation] TRP チャネル活性化後の ATP によって仲介される象牙芽細胞－象牙芽細胞情報伝達 (2014)
  • Author(s): 佐藤正樹、津村麻記、征矢学、川口綾、西山明宏、小倉一宏、望月浩幸、 児玉紗耶香、澁川義幸、田崎雅和
  • Organizer: 第91回日本生理学会大会
  • Place of Presentation: 鹿児島大学郡元キャンパス、鹿児島
• [Presentation] マウス由来象牙芽細胞系細胞におけるグルタミン酸受容体の機能発現 (2014)
  • Author(s): 西山明宏 、佐藤正樹 、津村麻記 、片倉朗 、田崎雅和 、澁川義幸
  • Organizer: 第91回日本生理学会大会
  • Place of Presentation: 鹿児島大学郡元キャンパス、鹿児島
• [Presentation] P2Y 12 -BK 受容体相互作用は cAMP に仲介される抑制効果を解除することで三叉神経節ニューロンに おけるリアノジン受容体誘発性 Ca2+ 放出を活性化する (2014)
  • Author(s): 川口綾、佐藤正樹、田崎雅和、一戸達也、澁川義幸
  • Organizer: 第91回日本生理学会大会
  • Place of Presentation: 鹿児島大学郡元キャンパス、鹿児島
• [Presentation] マウス由来象牙芽細胞における細胞膜伸展受容TRP チャネルとNa^<+>-Ca^<2+> exchanger の機能連関 (口頭発表) (2013)
  • Author(s): 佐藤正樹, 津村麻記, Sobhan Ubaidus, 児玉紗耶香, 隝田みゆき, 西山明宏, 望月浩幸, 小倉一宏, 田崎雅和, 澁川義幸
  • Organizer: 第55回歯科基礎医学会学術大会・総会
  • Place of Presentation: 岡山
• [Presentation] 象牙芽細胞におけるアルカリ感受性の検討 (ポスター発表) (2013)
  • Author(s): 津村麻記, 佐藤正樹, Sobhan Ubaidus, 児玉紗耶香, 隝田みゆき, 西山明宏, 田崎雅和, 澁川義幸
  • Organizer: 第55回歯科基礎医学会学術大会・総会
  • Place of Presentation: 岡山
• [Presentation] Guaiacol は象牙芽細胞のTRPV3 チャネルに作用する (ポスター発表) (2013)
  • Author(s): 隝田みゆき, 津村麻記, 佐藤正樹, Sobhan Ubaidus, 田崎雅和, 澁川義幸
  • Organizer: 第55回歯科基礎医学会学術大会・総会
  • Place of Presentation: 岡山
• [Presentation] Expression of TRP channels in isolated single Merkel cells from hamster oral mucosa (2013)
  • Author(s): Soya M., Kuroda H., Kawaguchi A, Sato M., Sobhan U., Tazaki M, Ichinohe T., Shibukawa Y
  • Organizer: 37th Congress of IUPS
  • Place of Presentation: UK, Birmingham
• [Presentation] Odontoblast as sensory receptor cell : TRP channels pannexin 1 and P2X_3 receptor coupling mediate sensory transduction in dentin(Oral) (2013)
  • Author(s): Shibukawa Y., Sato M., Tsumura M., Kuroda H., Sobhan U., Tazaki M
  • Organizer: 11th International Conference on Tooth Morphorogenesis and Differentiation (TMD2013)
  • Place of Presentation: France, La Londe Les Maures
• [Presentation] Expression of TRPM8 and TRPA1 channels in rat odontoblasts (2013)
  • Author(s): Sato M., Tsumura M, Sobhan U, Tazaki M, Shibukawa Y
  • Organizer: 11th International Conference on Tooth Morphorogenesis and Differentiation (TMD2013)
  • Place of Presentation: France, La Londe Les Maures
• [Presentation] マウス由来象牙芽細胞における細胞膜伸展受容TRPチャネルとNa+-Ca2+ exchenger の機能連関 (2013)
  • Author(s): 佐藤正樹、津村麻記、Sobhan Ubaidus、児玉紗耶香、隝田みゆき、西山明宏、望月浩幸、 小倉一宏、田崎雅和、澁川義幸
  • Organizer: 第55 回歯科基礎医学会学術大会・総会
  • Place of Presentation: 岡山コンベンションセンター、岡山
• [Presentation] 象牙芽細胞におけるアルカリ感受性の検討 (2013)
  • Author(s): 津村麻記、佐藤正樹、Sobhan Ubaidus、児玉紗耶香、隝田みゆき、西山明宏、 田崎雅和、澁川義幸
  • Organizer: 第55 回歯科基礎医学会学術大会・総会
  • Place of Presentation: 岡山コンベンションセンター、岡山
• [Presentation] Guaiacol は象牙芽細胞のTRPV3 チャネルに作用する (2013)
  • Author(s): 隝田みゆき、津村麻記、佐藤正樹、Sobhan Ubaidus、田崎雅和、澁川義幸
  • Organizer: 第55 回歯科基礎医学会学術大会・総会
  • Place of Presentation: 岡山コンベンションセンター、岡山
• [Presentation] Confocal microscope observation on paracellular fluid transport in the vascularly perfused submandibular gland. (2013)
  • Author(s): M. Murakami, F. Wei, T. Narita, M. Fukushima, S. Hashimoto, Y. Shibukawa, M. Sato.
  • Organizer: 37th Congress of IUPS
  • Place of Presentation: UK, Birmingham
• [Presentation] Expression of TRP channels in isolated single Merkel cells from hamster oral mucosa. (2013)
  • Author(s): M. Soya, H. Kuroda, A. Kawaguchi, M. Sato, U. Sobhan, M. Tazaki, T. Ichinohe, Y. Shibukawa.
  • Organizer: 37th Congress of IUPS
  • Place of Presentation: UK, Birmingham
• [Presentation] Odontoblast as sensory receptor cell: TRP channels, pannexin 1 and P2X3 receptor coupling mediate sensory transduction in dentin. (2013)
  • Author(s): Y. Shibukawa, M. Sato, M. Tsumura, H. Kuroda, U. Sobhan, M. Tazaki.
  • Organizer: 11th International Conference on Tooth Morphorogenesis and Differentiation (TMD2013)
  • Place of Presentation: France, La Londe Les Maures
• [Presentation] Expression of TRPM8 and TRPA1 channels in rat odontoblasts. (2013)
  • Author(s): M. Sato, M. Tsumura, U. Sobhan, M. Tazaki, Y. Shibukawa.
  • Organizer: 11th International Conference on Tooth Morphorogenesis and Differentiation (TMD2013)
  • Place of Presentation: France, La Londe Les Maures
• [Presentation] 象牙芽細胞におけるTRPM8 チャネルとTRPA1 チャネルの発現検索 oral presentation (2012)
  • Author(s): 津村麻記, Sobhan Ubaidus, 佐藤正樹, 西山明宏, 田崎雅和, 澁川義幸
  • Organizer: 第54回歯科基礎医学会
  • Place of Presentation: 郡山市
  • Year and Date: 2012-09-15
• [Presentation] Sodium-Calcium Exchangers in Rat Trigeminal Ganglion Neurons. poster presentation (2012)
  • Author(s): Kuroda H, Kawaguchi A, Soya M, Sato M., Sobhan U., Tsumura M., Tazaki M., Ichinohe T. and Shibukawa Y
  • Organizer: 第6回トランスポーター研究会九州部会
  • Place of Presentation: 福岡市
  • Year and Date: 2012-09-01
• [Presentation] 象牙芽細胞におけるTRPV1 チャネル・CB1 受容体・Na+-Ca2+交換体の機能連関 poster presentation (2012)
  • Author(s): 津村麻記, Sobhan Ubaidus, 佐藤正樹, 西山明宏, 田崎雅和, 澁川義幸
  • Organizer: 第6回トランスポーター研究会九州部会
  • Place of Presentation: 福岡市
  • Year and Date: 2012-09-01
• [Presentation] マウス象牙芽細胞系細胞における細胞膜伸展刺激受容TRP channels とNCX の機能関連 poster presentation (2012)
  • Author(s): 佐藤正樹, 津村麻記, Sobhan Ubaidus, 黒田英孝, 田崎雅和, 澁川義幸
  • Organizer: 第6回トランスポーター研究会九州部会
  • Place of Presentation: 福岡市
  • Year and Date: 2012-09-01
• [Presentation] 象牙芽細胞系細胞における機械刺激受容と三叉神経節細胞共培養系における伝達機構 Oral presentation (2012)
  • Author(s): Sato M., Tsumura M., Sobhan U, Kuroda H., Soya M., Masamura A., Tazaki M., Ihinohe T., Shibukawa Y
  • Organizer: 11th Kushiro Neuroscience Workshop
  • Place of Presentation: Kushiro, Hokkaido, Japan
• [Presentation] 象牙芽細胞におけるグルタミン酸受容体の発現Oral presentation (2012)
  • Author(s): Nishiyama A., Sato M., Shibukawa Y., Tazaki M, Katakura A
  • Organizer: 11th Kushiro Neuroscience Workshop
  • Place of Presentation: Kushiro, Hokkaido, Japan
• [Presentation] 象牙芽細胞系細胞における機械刺激受容と三叉神経節細胞共培養系における伝達機構 (2012)
  • Author(s): 佐藤正樹、津村麻記、Sobhan Ubaidus、黒田英孝、征矢学、正村綾、田﨑雅和、一戸達也、澁川義幸
  • Organizer: 第11回釧路ニューロサイエンスワークショップ
  • Place of Presentation: 釧路プリンスホテル
• [Presentation] マウス象牙芽細胞系細胞における細胞膜伸展刺激受容TRP channelsとNCXの機能関連 (2012)
  • Author(s): 佐藤正樹、津村麻記、Sobhan Ubaidus、黒田英孝、田﨑雅和、澁川義幸
  • Organizer: 第６回トランスポーター研究会九州部会
  • Place of Presentation: 福岡県歯科医師会館
• [Presentation] Activation of TRP channels by osmotic stimulation and Ca^<2+> extrusion by Na^<+>-Ca^<2+> exchangers in mouse odontoblast lineage cells (2011)
  • Author(s): M. Sato, U. Sobhan, M. Tsumura, H. Ichikawa, M.Tazaki, T. Muramatsu, Y. Shibukawa
  • Organizer: 6th International Conference on Sodium Calcium Exchanger
  • Place of Presentation: Naples, Italy
• [Presentation] NCX expression in enamel/dentin-forming cells. Poster presentation (2011)
  • Author(s): Shibukawa Y., Tsumura M., Sato M., Sobhan U., Muramatsu T., Matsuda T., Baba A., Tazaki M
  • Organizer: 6th International Conference on Sodium Calcium Exchanger 2011
  • Place of Presentation: Lacco Ameno, Ischia Island, Naples, Italy
• [Presentation] Functional coupling between TRPV1, CB1 and NCXs in rat odontoblasts. Poster presentation (33) (2011)
  • Author(s): Tsumura M., Shibukawa Y., Muramatsu T., Sobhan U., Sato M, Ichikawa H., Tazaki T
  • Organizer: 6th International Conference on Sodium Calcium Exchanger 2011
  • Place of Presentation: Lacco Ameno, Ischia Island, Naples, Italy
• [Presentation] ラット顎下腺細胞間分泌細管の微細形態: 急速凍結固定割断試料の電顕観察と高速共焦点レーザー顕微鏡観察
  • Author(s): 村上政隆、魏飛、成田貴則、福島美和子、橋本貞光、澁川義幸、佐藤正樹
  • Organizer: 第58回日本唾液腺学会
  • Place of Presentation: 文京学院大学、東京
• [Book] 日本歯科評論 2月号 (2013)
  • Author(s): 佐藤正樹、しま田みゆき、津村麻記、Sobhan Ubaidus、澁川義幸
  • Total Pages: 3
  • Publisher: （株）ヒョーロン・パブリッシャーズ
• [Book] 歯科医学の古きをたずねて新しきを知る 第2回: 歯内療法薬剤の作用機序 (2012)
  • Author(s): 佐藤正樹, 隝田みゆき, 津村麻記, Sobhan Ubaidus, 田崎雅和
  • Publisher: 日本歯科評論
• [Book] 歯髄・象牙質感覚の基礎と臨床, 日本歯科医師会雑誌 (2012)
  • Author(s): 澁川義幸, 市川秀樹, Sobhan Ubaidus, 津村麻記, 佐藤正樹, 田崎雅和