Analysis of the role of mechanosensor-mediated JNK/p38 signaling in osteoimmunological regulation of osteoblasts
Project/Area Number |
24792059
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Prosthetic dentistry
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Research Institution | Tohoku University |
Principal Investigator |
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Project Period (FY) |
2012-04-01 – 2014-03-31
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Project Status |
Completed (Fiscal Year 2013)
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Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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Keywords | メカノバイオロジー / 骨代謝 / JNK / p38 / ERK / メカノトランスダクション / ストレス応答MAPキナーゼ / 骨免疫学 |
Research Abstract |
In this study, we report that large-magnitude (12%) cyclic stretch induced Ca2+ influx in MC3T3-E1 osteoblasts, which subsequently activated ASK1 MAP3K. The activated ASK1-JNK lead to transiently enhanced expression of FGF-inducible 14 (Fn14, a member of the TNF receptor superfamily) gene. Cells with enhanced expression of Fn14 subsequently acquired sensitivity to the ligand of Fn14, TNF-related weak inducer of apoptosis, and underwent apoptosis. On the other hand, ASK1-p38 pathway induced expression of monocyte chemoattractant protein-3 (MCP-3) gene, which promoted chemotaxis of pre-osteoclasts. These results suggest that ASK1-JNK/p38 pathways contribute to the mechanism of mechanical overloading-induced bone resroption via cytokine-related gene expression.
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Report
(3 results)
Research Products
(9 results)
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[Journal Article] The expression of Fn14 via mechannical stress-activated JNK contributes to apoptosis induction in osteoblasts2014
Author(s)
Matsui H., Fukuno N., Kanda Y., Kantoh Y., Chida T., Nagaura Y., Suzuki O., Nishitoh H., Takeda K., Ichijo H., Sawada Y., Sasaki K., Kobayashi T., and Tamura S
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Journal Title
J. Biol. Chem
Volume: 289
Pages: 6438-6450
Related Report
Peer Reviewed
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[Journal Article] The expression of Fn14 via mechanical stress-activated JNK contributes to apoptosis induction in osteoblasts.2014
Author(s)
Matsui, H., Fukuno, N., Kanda, Y., Kantoh, Y., Chida, T., Nagaura, Y., Suzuki, O., Nishitoh, H., Takeda, K., Ichijo, H., Sawada, Y., Sasaki, K., Kobayashi, T. and Tamura, S.
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Journal Title
J. Biol. Chem.
Volume: 289
Issue: 10
Pages: 6438-6450
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] メカニカルストレスと変形性関節症2012
Author(s)
松井裕之, 原田伊知郎, 石島旨章, 澤田泰宏
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Journal Title
CLINICAL CALCIUM
Volume: Vol.22 No.12(doi:CliCa121218551862)
Pages: 1855-1862
Related Report
Peer Reviewed
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