| Project/Area Number |
24792078
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Prosthetic dentistry
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| Research Institution | Nagasaki University |
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Principal Investigator |
WATAMOTO Takao 長崎大学, 医歯(薬)学総合研究科, 助教 (60420444)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | 口腔細菌学 / バイオフィルム / カンジダ / 小分子化合物 / 抗真菌剤 / レジン床義歯 |
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| Research Abstract |
To develop new small-molecule antifungal compounds by library screening methods using Candida Albicans, and to evaluate their antifungal effects on Candida biofilms and cytotoxic effects on human cells. Wild-type C. albicans strain SC5314 was used in this study. To identify antifungal compounds, we screened a small-molecule library using an antifungal susceptibility test (AST). We tested the cytotoxicity of the hit compounds using human gingival fibroblast (hGF) cells to evaluate their clinical safety. Only 35 compounds were identified by screening, which inhibited the metabolic activity of C. albicans by >50%. Five compounds had strong fungicidal effects and could inhibit the metabolic activity of Candida biofilms. Only CV-3988 showed no cytotoxicity at a fungicidal concentration of 0.25M. CV-3988 had fungicidal effects on C. albicans, but low cytotoxic effects on hGF cells.
Therefore, CV-3988 could be a novel treatment for superficial mucosal candidiasis.
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