Investigation of biological pathogenesis and development of prevention diagnosis protocol of peri-implantitis.

• Project/Area Number: 24792083
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Prosthetic dentistry
• Research Institution: Okayama University
• Principal Investigator: MINO Takuya 岡山大学, 医歯(薬)学総合研究科, 助教 (10625718)
• Research Collaborator: KUBOKI Takuo ; SONOYAMA Wataru ; ONO Mitsuaki ; ONO Aya
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 口腔インプラント / インプラント周囲炎 / 生物学的リスクファクター / 次世代シークエンサー / 診断プロトコル / マイクロアレイ法 / メタゲノム解析 / 質量分析法

Outline of Final Research Achievements

The purpose of this study was to identify the biological markers that are involved in the onset and progression of peri-implantitis. First, we developed a clinical examination protocol by referring to the previous reports and investigated the prospective study for the initial peri-implantitis patient using the developed protocol. The subject of this study was five and, basic information of subjects was shown below, average age: 71.0 ± 4.6 years, average number of implants per patient : 4.8 ± 2.4, average number of implant with peri-implantitis：1.4 ± 0.5) . To identify the biological markers, gingival crevicular fluids around the oral implant and natural tooth were collected by using the paper points. ,The adequate amount of bacterial DNA could be collected for 16S rRNA sequencing using this protocol.

Research Products

• [Journal Article] <i>In silico</i> comparison of the reproducibility of full-arch implant provisional restorations to final restoration between a 3D Scan/CAD/CAM technique and the conventional method (2015)
  • Author(s): Mino T, Maekawa K, Ueda A, Higuchi S, Sejima J, Takeuchi T, Hara ES, Kimura-Ono A, Sonoyama W, Kuboki T.
  • Journal Title: Journal of Prosthodontic Research Volume: 59 Issue: 2 Pages: 152-158
  • DOI: 10.1016/j.jpor.2014.12.001
  • NAID: 130007936670
  • ISSN: 1883-1958, 1883-9207
  • Peer Reviewed
• [Presentation] OHRQoL Promotion was maintained even 6-Year after Implant-supported Prosthodontic Treatment. (2015)
  • Author(s): Kurosaki Y, Kimura-Ono A, Koyama E, Nakagawa S, Mino T, Maekawa K, Kuboki T
  • Organizer: 2015 IADR/AADR/CADR General Session & Exhibition program.
  • Place of Presentation: Boston, USA
  • Year and Date: 2015-03-14
• [Presentation] A computer-based morphological transfer method to replicate provisional to final implant superstructure. (2014)
  • Author(s): Mino T, Maekawa K, Ueda A, Higuchi S, Sejima J, Takeuchi T, Hara ES, Kimura-Ono A, Sonoyama W, Kuboki T
  • Organizer: Indonesia & Japan Prosthodontic Society Joint Meeting.
  • Place of Presentation: Bari, Indonesia
  • Year and Date: 2014-10-31
• [Presentation] 口腔インプラント周囲炎病巣の細菌叢のメタゲノム解析の相談． (2013)
  • Author(s): 前川賢治，大野充昭，小山絵里，大野 彩，三野卓哉， Emilio Hara Satoshi，園山 亘，黒﨑陽子，和田崇之，大原直也，窪木拓男.
  • Organizer: NGS現場の会第3回研究会.
  • Place of Presentation: 神戸，日本.
• [Presentation] 多変量解析を用いた即時荷重インプラント成功のリスク因子の同定. (2013)
  • Author(s): 高山賢一，荒川 光，三野卓哉，大野 彩，嶌岡英起，窪木拓男，桐田忠昭．
  • Organizer: 第43回公益社団法人日本口腔インプラント学会・学術大会.
  • Place of Presentation: 福岡，日本.
• [Presentation] 少数歯欠損患者における補綴治療後5年経過時の口腔関連Quality of Lifeの評価. (2013)
  • Author(s): 黒﨑陽子，大野 彩，瀧内博也，佐伯真未子，小山絵理，三野卓哉，前川賢治，窪木拓男.
  • Organizer: 公益社団法人日本口腔インプラント学会第34回中国・四国支部総会学術大会.
  • Place of Presentation: 鳥取, 日本.
• [Presentation] 多変量解析を用いたオッセオインテグレーション獲得のリスク因子の検討：即時荷重はリスク因子？ (2012)
  • Author(s): 松原有里，高山賢一，荒川 光，三野卓哉，桐田忠昭.
  • Organizer: 第42回公益社団法人日本口腔インプラント学会学術大会.
  • Place of Presentation: 大阪, 日本.
• [Book] スウェーデンの介護現場におけるインプラントに対する歯科医師の認識と現状 (2014)
  • Author(s): 三野卓哉，窪木拓男
  • Total Pages: 3
  • Publisher: 株式会社ヒョーロン・パブリッシャーズ