|Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
This study demonstrated the involvement of the T-box transcription factor Brachyury in early-stage embryonic mouse salivary gland development. RT-PCR and immunoblotting revealed that the expression of Brachyury increased in the SMG and peaked between E12.5-13.5, concomitant with the early stage of branching morphogenesis. In addition, fibronectin and Btbd7 (which is regulated by fibronectin), which are both essential for cleft formation, were expressed strongly during the same period. Furthermore, the Sox2 genes, which regulates cell growth, was also strongly expressed during E12.5-13.5. When Brachyury was knocked down in SMG rudiments in organ culture, cleft formation was suppressed, and branching morphogenesis was inhibited. The expression of Sox2, fibronectin and Btbd7 were also suppressed by knockdown of the Brachyury gene, suggesting that Brachyury plays a central role in regulating cell growth and cleft formation in early-stage embryonic mouse salivary gland development.