Stress-endureing cells from oral tissues for bone engineering

Research Project

Project/Area Number	24792145
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Dental engineering/Regenerative dentistry
Research Institution	Nagasaki University
Principal Investigator	TAJIMA Nobutaka 長崎大学, 大学病院, 助教 (00447492)
Project Period (FY)	2012-04-01 – 2014-03-31
Project Status	Completed (Fiscal Year 2013)
Budget Amount *help	¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000) Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000) Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords	骨再生 / ストレス耐性 / 間葉系幹細胞 / 骨再生医療 / 多能性幹細胞
Research Abstract	Human periodontal ligament cells were exposed to severe hypoxic (O2<0.1%) condition in culture to isolate the stress-enduring cells for bone engineering. Cells survived under this condition for 12 hours showed the high expression of pluripotent stem cell markers as well as mesenchymal stem cell markers, and also displayed the highest pluripotency in vitro. In particular, these surviving cells after re-oxygenation could form abundant calcified-nodules in osteogenic medium. Furthermore, a complex with these stress-enduring cells and fibrin-gel showed the remarkable osteoinducibility in vivo.