| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
|---|
| Outline of Final Research Achievements |
Implant abutment surface made by CAD/CAM technique have contaminants due to the adjusting and polishing process by dental technician. The aim of this study was to develop the cleaning methods of implant abutment, and to investigate the effect of decontamination on human gingival fibroblasts (HGFs). Newly developed washing reagent (PK) and/or vacuum plasma (Plasma) were applied for the cleaning of zirconia surface, and evaluated the effect of decontamination by X-ray photoelectron spectroscopy. HGFs were cultured on the sample for 6, 24, and 48h, and evaluated the attachment, gene expression of VEGFA, IL-6, and collagen. Contaminants on the surface reduced by washing with PK and PK+Plasma groups. Attachments of HGFs were also increased in PK and PK+Plasma groups. Gene expressions of IL-6 and VEGFA were reduced at 6 hours and 6, 24, 48 hours, respectively. Gene expression of collagen was increased from 6 hours, especially at 48 hours after seeding.
|
