| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Outline of Final Research Achievements |
We reported to establish the periodontal ligament (PDL) derived stem cells, which had the capacity to construct blood vessel structures. Thereafter, we observed these PDL cells expressed multiple hematopoietic stem cell markers and possessed the capacity to induce eosinophilic blood cells inside the constructed vessel lumen in our three-dimensional culture. To identify the vital genes associated with regulation of hemangioblastic capacity, we performed cDNA microarray analysis and then selected one transcription factor.
Subsequently, we constructed adenoviral vector including the sequence of selected gene and transfected the vector with the PDL cells. According to this treatment, both angiogenic and hematopoietic capacity were significantly enhanced. Futhermore, the gene expression of hematopoietic marker gene; c-kit(CD117) was upregulated in viral titer-dependent manner. In consequence of these data, we identified one vital gene to regulate hemangioblastic capacity of the PDL cells.
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