Establishment of neovascularization-inducing technology accompanied with hematopoiesis by utilizing Periodontal ligament derived stem cells
| Project/Area Number |
24792149
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Dental engineering/Regenerative dentistry
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| Research Institution | Hokkaido University |
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Principal Investigator |
OKUBO Naoto 北海道大学, 薬学研究科(研究院), 研究員 (00553207)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Keywords | 再生医療 / 造血幹細胞 / 血管新生 / 歯根膜幹細胞 / 三次元培養 |
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| Outline of Final Research Achievements |
We reported to establish the periodontal ligament (PDL) derived stem cells, which had the capacity to construct blood vessel structures. Thereafter, we observed these PDL cells expressed multiple hematopoietic stem cell markers and possessed the capacity to induce eosinophilic blood cells inside the constructed vessel lumen in our three-dimensional culture. To identify the vital genes associated with regulation of hemangioblastic capacity, we performed cDNA microarray analysis and then selected one transcription factor.
Subsequently, we constructed adenoviral vector including the sequence of selected gene and transfected the vector with the PDL cells. According to this treatment, both angiogenic and hematopoietic capacity were significantly enhanced. Futhermore, the gene expression of hematopoietic marker gene; c-kit(CD117) was upregulated in viral titer-dependent manner. In consequence of these data, we identified one vital gene to regulate hemangioblastic capacity of the PDL cells.
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Report
(4 results)
Research Products
(28 results)
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[Journal Article] Human Amnion-Derived Mesenchymal Stem Cell Transplantation Ameliorates Dextran Sulfate Sodium-Induced Severe Colitis in Rats.2015
Author(s)
Onishi R, Ohnishi S, Higashi R, Watari M, Yamahara K, Okubo N, Nakagawa K, Katsurada K, Suda G, Natsuizaka M, Takeda H, Sakamoto N
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Journal Title
Cell Transplantation
Volume: -
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Novel SCRG1/BST1 axis regulates self-renewal, migration, and osteogenic differentiation potential in mesenchymal stem cells.2014
Author(s)
Aomatsu, E., Takahashi, N., Sawada, S., Okubo, N., Hasegawa, T., Taira, M., Miura, H., Ishisaki, A., and Chosa, N.
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Journal Title
Sci. Rep.
Volume: 4
Issue: 1
Pages: 3652-3652
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] EGF positively regulates the proliferation and migration, and negatively regulates the myofibroblast differentiation of periodontal ligament-derived endothelial progenitor cells through MEK/ERK- and JNK-dependent signals.2013
Author(s)
Kimura, H., Okubo, N., Chosa, N., Kyakumoto, S., Kamo, M., Miura, H. and Ishisaki, A
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Journal Title
Cell. Physiol. Biochem.
Volume: 32
Issue: 4
Pages: 899-914
DOI
Related Report
Peer Reviewed
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[Journal Article] 六君子湯による摂食不振・悪液質の改善2013
Author(s)
武田 宏司, 中川 浩治, 西村 三恵, 大久保 直登, 細野 秀崇, 浅香 正博, 武藤 修一, 大西 俊介, 坂 本 直哉
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Journal Title
Jounal of Gastrointestinal Reserch
Volume: 21(1)
Pages: 48-58
Related Report
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[Journal Article] 六君子湯による摂食不振・悪液質の改善2013
Author(s)
武田 宏司, 中川 浩治, 西村 三恵, 大久保 直登, 細野 秀崇, 浅香 正博, 武藤 修一, 大西 俊介, 坂本 直哉
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Journal Title
Jounal of Gastrointestinal Reserch 2013;Vol21 no.1
Volume: Vol21 no.1
Pages: 48-58
Related Report
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