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Tooth and periodontal tissue regeneration with purified mesenchymal stem cells and gene transfer technique

Research Project

Project/Area Number 24792151
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Dental engineering/Regenerative dentistry
Research InstitutionTohoku University (2014-2015)
Keio University (2012-2013)

Principal Investigator

Niibe Kunimichi  東北大学, 大学病院, 助教 (50468500)

Project Period (FY) 2012-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Keywords間葉系幹細胞 / 歯胚再生 / 再生医療 / 歯原性上皮 / 歯原性間葉 / iPS細胞 / 歯牙再生 / 遺伝子導入
Outline of Final Research Achievements

The cultivable mesenchymal stem cell (MSC) has self-renewal competence. However, self-renewal ability of MSC shows a decrease after several passages using adhesion culture system. In addition, differentiation advancing and cell phenotype changing also appear in MSC after long term culture using the conventional adhesion culture. It is necessary to multiply the purified MSC in vitro without losing their stem-ness for their use in tooth germ reproduction. Therefore, establish a new culture method to maintain the stem-ness of the MSC is imperative.
Currently applicant to maintain the undifferentiated MSCs in a particular environment. Further, this culture method could be resurrected undifferentiated stage. This method can maintain the stem-ness of MSC through forming a three-dimensional cell mass. This research is delayed from the plan, but now, it is carried out to continue the research for the realization of the tooth germ and periodontal tissue regeneration using this cell mass.

Report

(5 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (13 results)

All 2015 2014 2013 2012

All Journal Article (5 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 5 results,  Open Access: 3 results) Presentation (8 results) (of which Int'l Joint Research: 1 results,  Invited: 2 results)

  • [Journal Article] Controlled Osteogenic Differentiation of Mouse Mesenchymal Stem Cells by Tetracycline-Controlled Transcreptional Activation of Amelogenin2015

    • Author(s)
      9.Wang F, Okawa H, Kamano Y, Niibe K, Kayashima H, Osathanon T, Pavasant P, Saeki M, Yatani H, Egusa H
    • Journal Title

      PloS One

      Volume: 10(12) Issue: 12 Pages: e0145677-e0145677

    • DOI

      10.1371/journal.pone.0145677

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Purified Human Dental Pulp Stem Cells Promote Osteogenic Regeneration.2015

    • Author(s)
      Yasui T, Mabuchi Y, Toriumi H, Ebine T, Niibe K, Houlihan DD, Morikawa S, Onizawa K, Kawana H, Akazawa C, Suzuki N, Nakagawa T, Okano H, Matsuzaki Y.
    • Journal Title

      journal of Dental Research

      Volume: 95 Issue: 2 Pages: 206-214

    • DOI

      10.1177/0022034515610748

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Osteonecrosis of the jaw in patients with dental prostheses being treated with bisphosphonates or denosumab2015

    • Author(s)
      Niibe K, Ouchi T, Iwasaki R, Nakagawa T, Horie N.
    • Journal Title

      Journal of Prosthodontic Research

      Volume: 59 Issue: 1 Pages: 3-5

    • DOI

      10.1016/j.jpor.2014.08.001

    • NAID

      130007936677

    • ISSN
      1883-1958, 1883-9207
    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] LNGFR(+)THY-1(+)VCAM-1(hi+) Cells Reveal Functionally Distinct Subpopulations in Mesenchymal Stem Cells.2013

    • Author(s)
      1. Mabuchi Y, Morikawa S, Harada S, Niibe K, Suzuki S, Renault-Mihara F, Houlihan DD, Akazawa C, Okano H, Matsuzaki Y
    • Journal Title

      Stem Cell Reports.

      Volume: 1(2) Pages: 152-165

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Isolation of mouse mesenchymal stem cells on the basis of expression of Sca-1 and PDGFR-α2012

    • Author(s)
      Houlihan DD, Mabuchi Y, Morikawa S, Niibe K, Araki D, Suzuki S, Okano H, Matsuzaki Y.(These authors contributed equally to this work.)
    • Journal Title

      Nat Protoc

      Volume: 7(12) Issue: 12 Pages: 2103-2111

    • DOI

      10.1038/nprot.2012.125

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] 6.Potential of purified mesenchymal stem cells for regenerative dentistry2015

    • Author(s)
      Kunimichi Niibe
    • Organizer
      Chulalongkorn-Tohoku Joint Symposium in Dental Science 2015
    • Place of Presentation
      タイ バンコク
    • Year and Date
      2015-12-09
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 2.「再生歯科補綴」の技術確立に向けて ―補綴歯科治療に求められる歯・歯槽骨の再生とは-2015

    • Author(s)
      新部邦透
    • Organizer
      第124回日本補綴歯科学会
    • Place of Presentation
      大宮ソニックシティ・大宮市
    • Year and Date
      2015-05-30
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 間葉系幹細胞純化技術の再生歯科医療への応用 (Application of purified mesenchymal stem cells to regenerative dentistry)2015

    • Author(s)
      新部邦透,江草宏
    • Organizer
      第4回補綴若手研究会
    • Place of Presentation
      熱海ホテルニューアカオ,熱海
    • Year and Date
      2015-03-21 – 2015-03-22
    • Related Report
      2014 Research-status Report
  • [Presentation] ヒトiPS細胞由来神経堤細胞群における効率的な純化間葉系幹細胞の回収2015

    • Author(s)
      黄地健仁,森川暁,新部邦透,奥野博庸,赤松和土,中川種昭,岡野栄之
    • Organizer
      第14回日本再生医療学会総会
    • Place of Presentation
      パシフィコ横浜,横浜
    • Year and Date
      2015-03-19 – 2015-03-21
    • Related Report
      2014 Research-status Report
  • [Presentation] 欠損歯補綴物を有するビスフォスフォネート製剤及びデノスマブ投与者の臨床統計2014

    • Author(s)
      黄地健仁,新部邦透,岩崎良太郎,有馬誠亮,龍留美子,鈴木啓介,鈴木潔,中川種昭,堀江伸行
    • Organizer
      公益社団法人日本補綴歯科学会東京支部総会第18回学術大会
    • Place of Presentation
      昭和大学旗の台キャンパス,東京
    • Year and Date
      2014-11-09
    • Related Report
      2014 Research-status Report
  • [Presentation] 中胚葉系細胞を起源とする間葉系幹細胞の分化能2013

    • Author(s)
      新部邦透, 森川暁, 中川種昭
    • Organizer
      第67回NPO法人日本口腔科学会学術集会
    • Place of Presentation
      宇都宮
    • Related Report
      2013 Research-status Report
  • [Presentation] ヒト間葉系幹細胞の予期的分離と機能解析2013

    • Author(s)
      森川暁, 馬淵洋, 新部邦透, 松崎有未, 岡野栄之, 河奈裕正, 中川種昭
    • Organizer
      第67回NPO法人日本口腔科学会学術集会
    • Place of Presentation
      宇都宮
    • Related Report
      2013 Research-status Report
  • [Presentation] 臨床応用へ向けた間葉系幹細胞の分離とiPS細胞の高品質化2012

    • Author(s)
      新部 邦透、堀江 伸行、鈴木 潔、鈴木 啓介、池田 浩子、西山 留美子、有馬   誠亮、中川 種昭
    • Organizer
      社団法人日本補綴歯科学会第121回学術大会
    • Place of Presentation
      横浜
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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