Cryopreservation for The Cell Selection Using Water Channels AQPs Combination with Ultra-quick Freezing
Project/Area Number |
24792152
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Dental engineering/Regenerative dentistry
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Research Institution | Keio University |
Principal Investigator |
YASUHIRO Kato 慶應義塾大学, 医学部, 助教 (40398780)
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Project Period (FY) |
2012-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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Keywords | 幹細胞 / アクアポリン / 凍結 / バイオ関連機器 |
Outline of Final Research Achievements |
Here we show that membrane damage caused by ultra-quick freezing is rescued by the expression of AQP4. We next examine if the expression of AQP4 combined with ultra-quick freezing can bes used to select cells efficiently under freezing conditions where most cells are died. Finally we propose that this simple but efficient and safe method may be applicable to the selection of mammalian cells for applications in regenerative medicine as well as cell-based functional assays or drug screening protocols.
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Report
(4 results)
Research Products
(8 results)
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[Journal Article] Unprecedented cell-selection using ultra-quick freezing combined with aquaporin expression.2014
Author(s)
Kato, Y., T. Miyauchi, Y. Abe, D. Koji´c, M. Tanaka, N. Chikazawa, Y. Nakatake, S. B. H. Ko, D. Kobayashi, A. Hazama, S. Fujiwara, T. Uchida, M. Yasui
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Journal Title
PLoS ONE
Volume: 9
Issue: 2
Pages: e87644-e87644
DOI
Related Report
Peer Reviewed / Open Access
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