Cryopreservation for The Cell Selection Using Water Channels AQPs Combination with Ultra-quick Freezing

• Project/Area Number: 24792152
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Dental engineering/Regenerative dentistry
• Research Institution: Keio University
• Principal Investigator: YASUHIRO Kato 慶應義塾大学, 医学部, 助教 (40398780)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 幹細胞 / アクアポリン / 凍結 / バイオ関連機器

Outline of Final Research Achievements

Here we show that membrane damage caused by ultra-quick freezing is rescued by the expression of AQP4. We next examine if the expression of AQP4 combined with ultra-quick freezing can bes used to select cells efficiently under freezing conditions where most cells are died. Finally we propose that this simple but efficient and safe method may be applicable to the selection of mammalian cells for applications in regenerative medicine as well as cell-based functional assays or drug screening protocols.

Research Products

• [Journal Article] Expression and localization of aquaporin-4 in sensory ganglia (2014)
  • Author(s): J Kato, Y Takai, M K Hayashi, Y Kato, M Tanaka, Y Soma, Y Abe, M Yasui
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 451 Issue: 4 Pages: 562-567
  • DOI: 10.1016/j.bbrc.2014.08.026
  • Peer Reviewed
• [Journal Article] Unprecedented cell-selection using ultra-quick freezing combined with aquaporin expression. (2014)
  • Author(s): Kato, Y., T. Miyauchi, Y. Abe, D. Koji´c, M. Tanaka, N. Chikazawa, Y. Nakatake, S. B. H. Ko, D. Kobayashi, A. Hazama, S. Fujiwara, T. Uchida, M. Yasui
  • Journal Title: PLoS ONE Volume: 9 Issue: 2 Pages: e87644-e87644
  • DOI: 10.1371/journal.pone.0087644
  • Peer Reviewed / Open Access
• [Presentation] 細胞選別を目的とした超急速凍結法 (2014)
  • Author(s): 加藤靖浩、宮内崇行、阿部陽一郎、田中愛美、安井正人
  • Organizer: 日本生化学会
  • Place of Presentation: 国立京都国際会館（京都）
  • Year and Date: 2014-10-16 – 2014-10-18
• [Presentation] 水分子動態を制御するアクアポリン発現と超急速凍結による細胞選択 (2014)
  • Author(s): 加藤靖浩、田中愛美、阿部陽一郎、中武悠樹、近澤奈々、洪繁、洪実、安井正人
  • Organizer: 発達腎研究会
  • Place of Presentation: 慶應義塾大学（東京）
  • Year and Date: 2014-08-31
• [Presentation] Cryopreservation for The Cell Selection Using Water Channels AQPs Combination with Ultra-quick Freezing (2014)
  • Author(s): Yasuhiro Kato
  • Organizer: Biotechnology2014 国際学会
  • Place of Presentation: バレンシア（スペイン）
  • Year and Date: 2014-06-24 – 2014-06-28
• [Presentation] 細胞選別を目的とした超急速凍結法 (2014)
  • Author(s): 加藤靖浩、宮内崇行、阿部陽一郎、田中愛美、安井正人
  • Organizer: 第87回日本薬理学会年会
  • Place of Presentation: 仙台国際センター
• [Presentation] Investigated freezing methods from water dynamics by aquaporin
  • Author(s): Yasuhiro Kato, Manami Tanaka, Youichiro Abe, Masato Yasui
  • Organizer: FEBS2012
  • Place of Presentation: セビリア（スペイン）
• [Presentation] 水分子動態を制御するアクアポリンから探る凍結法
  • Author(s): 加藤靖浩、田中愛美、阿部陽一郎、安井正人
  • Organizer: 日本生化学会
  • Place of Presentation: 福岡