Evaluation of in vivo tissue forming potential and homing of mesenchymal stem cells derived from human extracted teeth and bone marrow.
| Project/Area Number |
24792157
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Dental engineering/Regenerative dentistry
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| Research Institution | The Nippon Dental University |
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Principal Investigator |
TAMAKI Yuichi 日本歯科大学, 生命歯学部, 助教 (10609457)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
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| Keywords | 抜去歯幹細胞 / 再生医学 / 硬組織形成能 / テロメアーゼ活性 / 幹細胞 / ヒト抜去歯 / 組織形成能 / 歯小嚢幹細胞 / 歯乳頭幹細胞 / 歯根膜幹細胞 / 歯髄幹細胞 / 骨髄幹細胞 |
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| Research Abstract |
We isolated and characterized dental stem cells (DSCs) from the dental pulp(DPSCs), periodontal ligament (PDLSCs), apical papilla (APSCs) and dental follicle (DFSCs) of mature and immature teeth, along with bone marrow-derived stem cells (BMSCs) from the iliac crest. This study compared the in vivo tissue forming capacities and proliferative potentials of these cells in terms of growth kinetics, telomerase activity. It was found that all kind of stem cells have hard tissue forming potential. In addition, it was demonstrated that DSCs possess and maintain high proliferative ability and retarded cellular senescence through long-term culture when compared with BMSCs. Our results provide new insights into the physiological properties of stem cells and indicate that DSCs are appropriate for basic research and clinical applications.
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Report
(3 results)
Research Products
(29 results)
