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Identification of immunomodulatory factors of dental pulp stem cells aiming at the pulp regeneration by allogeneic transplantation

Research Project

Project/Area Number 24792164
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Dental engineering/Regenerative dentistry
Research InstitutionNational Center for Geriatrics and Gerontology

Principal Investigator

MURAKAMI Masashi  独立行政法人国立長寿医療研究センター, 再生歯科医療研究部, 研究員 (30614531)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords歯髄幹細胞 / 歯髄幹細胞培養上清 / 混合リンパ球培養反応 / 同種移植 / 免疫制御 / 培養上清 / 歯髄再生 / リンパ球混合培養反応
Outline of Final Research Achievements

The conditioned medium (CM) of dental pulp stem cells (DPSCs) was found to be the most effective in immunomodulation compared to the CM of bone marrow stem cells (BMSCs) and adipose stem cells (ADSCs). We investigated the immunomodulatory factors present in the CM of DPSCs compared to those of BMSCs and ADSCs using microarray data of pulp, BM, and adipose CD31- SP cells. The highest expressed factors in pulp CD31- SP cells compared to BM and adipose CD31- SP cells were further investigated using Real-time RT-PCR and Milliplex analysis.
MCP-1 (monocyte chemoattractant protein-1) level was higher in DPSCs CM than any other chemokine or cytokine. Moreover, MCP-1 suppressed the inflammatory response induced by IFN-γ and LPS in vitro. All these results suggested that MCP-1 was involved in the immunomodulatory effect of the CM of DPSCs.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (9 results)

All 2014 2013 2012

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (5 results)

  • [Journal Article] A novel combinatorial therapy with pulp stem cells and granulocyte colony-stimulating factor for total pulp regeneration2013

    • Author(s)
      Koichiro Iohara, Masashi Murakami, Norio Takeuchi, Yohei Osako, Masataka Ito, Ryo Ishizaka, Shinji Utsunomiya, Hiroshi Nakamura, Kenji Matsushita, Misako Nakashima
    • Journal Title

      Stem cells translational medicine

      Volume: 2 Issue: 7 Pages: 521-533

    • DOI

      10.5966/sctm.2012-0132

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] The use of granulocyte-colony stimulating factor induced mobilization for isolation of dental pulp stem cells with high regenerative potential.2013

    • Author(s)
      Murakami M, Horibe H, Iohara K, Hayashi Y, Osako Y, Takei Y, Nakata K, Motoyama N, Kurita K, Nakashima M.
    • Journal Title

      Biomaterials

      Volume: 34 Issue: 36 Pages: 9036-9047

    • DOI

      10.1016/j.biomaterials.2013.08.011

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Stimulation of angiogenesis, neurogenesis and regeneration by side population cells from dental pulp2013

    • Author(s)
      Ryo Ishizaka, Yuki Hayashi, Koichiro Iohara, Masahiko Sugiyama, Masashi Murakami, Tsubasa Yamamoto, Osamu Fukuta, Misako Nakashima
    • Journal Title

      Biomaterials

      Volume: 34 Issue: 8 Pages: 1888-1897

    • DOI

      10.1016/j.biomaterials.2012.10.045

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] GMP準拠にて製造加工したヒト臨床グレード歯髄幹細胞の品質・安全性の証明2013

    • Author(s)
      庵原耕一郎、村上真史、武井佳史、堀部宏茂、栗田賢一、中村洋、中島美砂子
    • Journal Title

      日本歯科保存学会誌

      Volume: 56 Pages: 121-129

    • NAID

      110009674420

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] イヌ歯髄、骨髄、脂肪幹細胞の抜髄後根管内自家移植による歯髄再生能の比較2014

    • Author(s)
      村上真史、林勇輝、庵原耕一郎、竹内教雄、中島美砂子
    • Organizer
      日本歯科保存学会 2014年度春季学術大会(第140回)
    • Place of Presentation
      滋賀県立芸術劇場
    • Year and Date
      2014-06-19 – 2014-06-20
    • Related Report
      2014 Annual Research Report
  • [Presentation] 優れた安全性と再生能を有するヒト歯髄幹細胞の分取法の開発2013

    • Author(s)
      村上真史、堀部宏茂、庵原耕一郎、林勇輝、大迫洋平、石坂亮、竹内教雄、栗田賢一、中島美砂子
    • Organizer
      第60回日本臨床検査医学会
    • Place of Presentation
      神戸国際会議場
    • Related Report
      2013 Research-status Report
  • [Presentation] 新規膜遊走分取法を用いたヒト歯髄幹細胞の特徴化と歯髄再生に対する有用性の検討2013

    • Author(s)
      村上真史、堀部宏茂、庵原耕一郎、林勇輝、大迫洋平、石坂亮、竹内教雄、栗田賢一、中島美砂子
    • Organizer
      第12回日本再生医療学会総会
    • Place of Presentation
      横浜
    • Related Report
      2012 Research-status Report
  • [Presentation] ブタ同一個体由来の歯髄、骨髄、脂肪幹細胞の形質の比較2012

    • Author(s)
      村上真史、庵原耕一郎、石坂亮、林勇輝、竹内教雄、中島美砂子
    • Organizer
      第11回日本再生医療学会総会
    • Place of Presentation
      横浜
    • Related Report
      2012 Research-status Report
  • [Presentation] 新規幹細胞分取法を用いたヒト歯髄幹細胞の特徴化と歯髄再生能2012

    • Author(s)
      村上真史、堀部宏茂、庵原耕一郎、石坂亮、林勇輝、竹内教雄、栗田賢一、中村洋、中島美砂子
    • Organizer
      第136回日本歯科保存学会
    • Place of Presentation
      宜野湾
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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