| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2012: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
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| Outline of Final Research Achievements |
Background: Nerve injury and consequence inflammatory responses produced by surgical incision result in a complicated pain status which still embarrasse half of all surgical patients. The aim of this study was to investigate whether stabilization of mast cells causes antinociceptive effects in a mouse postoperative pain model.
Methods: The experiments were conducted using BALB/c mice. The postoperative pain model was achieved by the incision of the hind paw. The mast cell membrane stabilizer cromoglycate (200 μg/20 μl) was injected before the incision in the hind paw and postoperative pain responses were assessed with guarding behaviors, withdrawal threshold to mechanical stimuli and latencies to heat. Pain behaviors were measured 1 day, 2 days, and 7 days after incision.
Results:Cromoglycate decreased guarding pain scores and withdrawal threshold to mechanical stimuli, however, withdrawal latencies to heat were not affected by the treatment of cromoglycate.
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