| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
The cellular tropisms of eukaryotic viruses are shaped by their need for entry receptors and intracellular transcription factors. Here we show that viral tropisms can also be regulated by tissue-specific microRNAs (miRNA). Since miRNA125b and miR100 are downregulated in oral cancer but highly expressed in normal tissue, we engineered a microRNA-sensitive virus containing target sites for those microRNAs in the 3′-untranslated region of an oncolytic Sindbis virus. The recombinant virus still propagated in cancer cells but could not replicate in cells expressing complementary miRNAs, suggesting a new modality of replicating viruses for cancer therapy.
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