Development of MicroRNA-sensitive Oncolytic Sindbis virus for Oral-cancer therapy
| Project/Area Number |
24792175
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Surgical dentistry
|
|---|
| Research Institution | Chiba University |
|---|
Principal Investigator |
SAITO KENGO 千葉大学, 医学(系)研究科(研究院), 講師 (70451755)
|
|---|
| Project Period (FY) |
2012-04-01 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
|---|
| Keywords | 腫瘍溶解性ウイルス / microRNA / 口腔癌 / シンドビスウイルス / マイクロRNA / 癌 |
|---|
| Outline of Final Research Achievements |
The cellular tropisms of eukaryotic viruses are shaped by their need for entry receptors and intracellular transcription factors. Here we show that viral tropisms can also be regulated by tissue-specific microRNAs (miRNA). Since miRNA125b and miR100 are downregulated in oral cancer but highly expressed in normal tissue, we engineered a microRNA-sensitive virus containing target sites for those microRNAs in the 3′-untranslated region of an oncolytic Sindbis virus. The recombinant virus still propagated in cancer cells but could not replicate in cells expressing complementary miRNAs, suggesting a new modality of replicating viruses for cancer therapy.
|
Report
(4 results)
Research Products
(5 results)
-
[Journal Article] Suppression of La Antigen Exerts Potential Antiviral Effects against Hepatitis A Virus.2014
Author(s)
Jiang X, Kanda T, Wu S, Nakamoto S, Saito K, Shirasawa H, Kiyohara T, Ishii K, Wakita T, Okamoto H, Yokosuka O.
-
Journal Title
PLoS One.
Volume: 9
Issue: 7
Pages: e101993-e101993
DOI
Related Report
Peer Reviewed / Open Access
-
[Journal Article] Reactivation of hepatitis B virus in hematopoietic stem cell transplant recipients in Japan: efficacy of nucleos(t)ide analogues for prevention and treatment.2014
Author(s)
Nakamoto S, Kanda T, Nakaseko C, Sakaida E, Ohwada C, Takeuchi M, Takeda Y, Mimura N, Iseki T, Wu S, Arai M, Imazeki F, Saito K, Shirasawa H, Yokosuka O.
-
Journal Title
Int J Mol Sci
Volume: 15
Issue: 11
Pages: 21455-67
DOI
Related Report
Peer Reviewed / Open Access
-
-
-
