Quantitative proteomics using formalin-fixed paraffin-embedded tissues of salivary gland cancer

• Project/Area Number: 24792179
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Surgical dentistry
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: NEGISHI Ayako 東京医科歯科大学, 歯学部附属病院, 非常勤講師 (70549972)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: プロテオーム / 口腔がん / バイオマーカー / プロテオミクス

Research Abstract

To identify prognostic factor of salivary gland cancers, the protein expression profiles of formalin-fixed paraffin-embedded (FFPE) tissue samples from salivary gland cancer patients were analyzed by quantitative proteomics. However, the histon proteins, abundant proteins, were mainly detected, and the expected protein markers were not detected. Therefore, we have changed the methods for extraction of peptides and performed quantitative proteomics for oral squamous cell carcinoma samples in which we also detected many abundant proteins. Then, we identified galectin-7 as a potential predictive marker of chemotherapy and/or radiotherapy resistance.

Research Products

• [Journal Article] Galectin-7 as a potential predictive marker of chemo- and/or radio-therapy resistance in oral squamous cell carcinoma. (2014)
  • Author(s): Matsukawa S, Morita K, Negishi A, Harada H, Nakajima Y, Shimamoto H, Tomioka H, Tanaka K, Ono M, Yamada T, Omura K.
  • Journal Title: Cancer Medicine Volume: 3(2) Issue: 2 Pages: 349-361
  • DOI: 10.1002/cam4.195
  • Peer Reviewed
• [Presentation] The potential predictive marker of chemotherapy and/or radiotherapy resistance in patients with oral SCC (2013)
  • Author(s): 松川 祥, 森田圭一, 根岸綾子, 原田浩之, 中島雄介, 島本裕彰, 富岡寛文, 田中香衣, 尾野雅哉, 山田哲司, 小村 健
  • Organizer: 第72回日本癌学会学術総会
  • Place of Presentation: 横浜市
• [Presentation] 定量的プロテオーム解析による口腔扁平上皮癌の術前治療抵抗性予測因子の同定 (2013)
  • Author(s): 松川 祥, 森田圭一, 根岸綾子, 原田浩之, 中島雄介, 島本裕彰, 富岡寛文, 田中香衣, 尾野雅哉, 山田哲司, 小村 健
  • Organizer: 第37回日本頭頸部癌学会
  • Place of Presentation: 東京
• [Presentation] 口腔癌のFFPEサンプルを用いた化学療法感受性予測因子に関するプロテオーム解析 (2013)
  • Author(s): 松川 祥, 森田圭一, 根岸綾子, 尾野雅哉, 山田哲司, 小村 健
  • Organizer: 第31回口腔腫瘍学会総会
  • Place of Presentation: 東京
• [Presentation] 口腔癌のFFPEサンプルを用いた化学療法感受性予測因子に関するプロテオーム解析 (2013)
  • Author(s): 松川 祥、森田 圭一、根岸 綾子、尾野 雅哉、山田 哲司、小村 健
  • Organizer: 第31回日本口腔腫瘍学会・学術大会
  • Place of Presentation: 秋葉原コンベンションホール