Gelatin hydrogel as a carrier of FGF during rat mandibular distraction
| Project/Area Number |
24792182
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Surgical dentistry
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
KABASAWA Yuji 東京医科歯科大学, 医歯(薬)学総合研究科, 講師 (10396963)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 口腔外科 / 骨延長 / ゼラチンハイドロゲル / FGF / 骨形成 / 成長因子 / bFGF / bFGFの臨床応用 / 顎骨延長法 |
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| Research Abstract |
Purpose: The aim of the present study was to evaluate the feasibility of a gelatin hydrogel system to enhance recombinant human fibroblast growth factor-2 (rhFGF-2)–induced osteogenic effects during rat mandibular distraction.
Materials and Methods: Mandibular distraction was performed in 28 male Wistar rats. Then, the rats were divided into 5 groups in which the designated gel mix was inserted into the distracted area: group 1, rhFGF-2 alone (n = 5); group 2, collagen alone (n = 6); group 3, collagen incorporating rhFGF-2 (n =6); group 4, gelatin hydrogel alone (n = 5); and group 5, gelatin hydrogel incorporating rhFGF-2 (n = 6).Results: Peripheral quantitative computed tomographic analysis, von Kossa staining, and calcein staining showed that using gelatin hydrogel with rhFGF-2 (group 5) significantly increased cortical bone mineraldensity, Group 5 also had a significantly larger number of cells compared with group 3 and radiopaque areas were observed morefrequently.
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Report
(3 results)
Research Products
(18 results)
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[Presentation] The feasibility of gelatin hydrogel as a carrier of basic fibroblast growth factor for bone formation in rat mandibular distraction2014
Author(s)
Yuhji Kabasawa, Atsushi Kimura, Masaru Sato, Namiaki Takahara, Kanako Matsumoto, Yusuke Higuchi, Yasuhiko Tabata, Kazuhiro Aoki, Keiichi Ohya, Ken Omura
Organizer
22nd Congress of the European Association for Cranio-Maxillo-Facial Surgery
Place of Presentation
Prague, Czech Republic (Praque)
Related Report
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[Presentation] ラット下顎骨延長モデルを用いたbasic fibroblast growth fatcor含有ゼラチンハイドロゲルの有用性評価
Author(s)
木村 敦, 樺沢勇司, 佐藤 昌, 高原楠旻, 樋口佑輔, 加藤玄樹, 菅森泰隆, 高橋真理子, 田村幸彦, 青木和広, 大谷啓一, 小村 健
Organizer
第33回日本歯科薬物療法学会
Place of Presentation
東京医科歯科大学
Related Report
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