| Project/Area Number |
24792189
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Surgical dentistry
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| Research Institution | Niigata University |
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Principal Investigator |
OHNUKI Hisashi 新潟大学, 医歯(薬)学総合研究科, 教務補佐員 (90568552)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | BRONJ / 口腔粘膜上皮細胞 / DNA損傷 / ビタミンB3 |
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| Research Abstract |
As one of the onset mechanisms of the bisphosphonate-related osteonecrosis of the jaw (BRONJ), zoledronic acid (ZOL) induced a DNA damage of oral keratinocytes and resulting in cell cycle S-phase arrest and repressive effects on cell viability, proliferation, and epithelial turnover.Niacin and the nicotinamide which are water soluble vitamin B3 are associated with a DNA repair mechanism. Therefore, we hoped that a DNA damage of oral keratinocytes was recovered by adding vitamins B3, and the proliferation potency of oral keratinocytes was restored. In this study, the niacin, nicotinamide and DMSO could not recovered the cellular proliferation. The keratinocytes were caused severe DNA damage by adding ZOL, suggesting that it was difficult to recover the DNA repairing.
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