Suppressive effect of invasion and metastasis of oral squamous cell carcinoma by Claudin expression

• Project/Area Number: 24792194
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Surgical dentistry
• Research Institution: Kanazawa University
• Principal Investigator: YOSHIZAWA Kunio 金沢大学, 大学病院, 講師 (60452108)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 臨床腫瘍学 / Claudin / Ｔｉｇｈｉｔ Ｊｕｎｃｔｉｏｎ / 口腔扁平上皮癌 / 浸潤 / 転移 / 山本・小浜分類 / 免疫組織化学的染色 / Claudin-7 / 口腔扁平上皮がん / 細胞接着

Research Abstract

Using immunohistochemical techniques to examine the expression levels of claudin-7 in 67 cases of OSCC, claudin-7 expression was detected in 35 (52.2%) of the 67 cases. Moreover, six cell lines with various invasive properties were investigated in vitro to compare mRNA and protein levels of claudin-7 using reverse transcription-polymerase chain reaction (RT-PCR) and the western blotting method. Decreased claudin-7 expression correlated significantly with T-category (p<0.05), lymph node metastasis (p<0.01), and mode of invasion (p<0.001). Patients with positive claudin-7 expression had a significantly better prognosis (p<0.05). Claudin-7 protein and mRNA levels were lower in the HOC313 and TSU cells, which have higher invasive potentials compared with other cell lines. These results suggest that loss of claudin-7 expression is associated closely with invasion and lymph metastasis and is an unfavorable prognostic factor in patients with OSCC.

Research Products

• [Journal Article] Loss of claudin-7 is a negative factor for invasion and metastasis in oral squamous cell carcinoma (2013)
  • Author(s): Yoshizawa K, Nozaki S, Kato A, Hirai M, Yanase M, Yoshimoto T, Kimura I, Sugiura S, Okamune A, Kitahara H, Noguchi N, Kato K, Ueki K, Kawashiri S
  • Journal Title: Oncology Reports Volume: 29 Pages: 445-450
  • Peer Reviewed
• [Journal Article] xCT inhibition depletes CD44v-expressing tum or cells that are resistant to EGFR-targeted therapy in head and neck squamous cellcarcinoma (2013)
  • Author(s): Yoshikawa M, Tsuchihashi K, Ishimoto T, Yae T, Motohara T, Sugihara E, O nishi N, Masuko T, Yoshizawa K, Kawashiri S, Mukai M, Asoda S, Kawana H, Nakagawa T, Saya H, Nagano O
  • Journal Title: Cancer Res Volume: 73 Pages: 1855-1866
  • Peer Reviewed
• [Journal Article] Loss of claudin-7 is a negative prognostic factor for invasion andmetastasis in oral squamous cell carcinoma (2013)
  • Author(s): Yoshizawa K., Nozaki S., Kato A., Hirai M., Yanase M., Yoshimoto T., KimuraI., Sugiura .S, Okamune A., Kitahara H., Noguchi N., Kato K., Ueki K., Kawashiri S
  • Journal Title: Oncology Report Volume: 29 Issue: 2 Pages: 445-450
  • DOI: 10.3892/or.2012.2161
  • Peer Reviewed
• [Journal Article] xCT Inhibition Depletes CD44v-Expressing Tumor Cells That Are Resistant to EGFR-Targeted Therapy in Head and Neck Squamous Cell Carcinoma (2013)
  • Author(s): Yoshikawa M, Tsuchihashi K, Ishimoto T, Yae T, Motohara T, Sugihara E, Onishi N, Masuko T, Yoshizawa K, Kawashiri S, Mukai M, Asoda S, Kawana H, Nakagawa T, Saya H, Nagano O
  • Journal Title: Cancer Res Volume: 73(6) Issue: 6 Pages: 1855-1866
  • DOI: 10.1158/0008-5472.can-12-3609-t
  • Peer Reviewed
• [Presentation] Expression of various canceration-related proteins on highly invasive oral squamous cell carcinomas showing type 4D (YK criteria: mode of invasion) (2014)
  • Author(s): Kunio Yoshizawa, Hiroko Kitahara, Natsuyo Noguchi, Koroku Kato, Hiroyuki Nakamura, Shuichi Kawashiri
  • Organizer: European congress on head & neck oncology 2014
  • Place of Presentation: Livapool (UK)
• [Presentation] 口腔扁平上皮癌の浸潤先進部のおけるCaveolin-1 発現に関する免疫組織化学的検討 (2013)
  • Author(s): 加藤広禄, 木村依世, 柳瀬瑞希, 杉浦史郎, 岡宗絢子, 北原寛子, 野口夏代, 吉澤邦夫, 中村博幸, 川尻秀一
  • Organizer: 第58回日本口腔外科学会
  • Place of Presentation: 福岡国際会議場 (福岡)
• [Presentation] 口腔扁平上皮がん浸潤様式 4D 型におけるがん悪性化に関わる各遺伝子発現についての検討 (2013)
  • Author(s): 吉澤邦夫, 北原寛子, 野口夏代, 加藤広禄, 中村博幸, 川尻秀一
  • Organizer: 第37回日本頭頸部癌学会
  • Place of Presentation: 京王プラザホテル(東京)
• [Presentation] 口腔扁平上皮がん浸潤様式4D型におけるがん悪性化に関わる各遺伝子発現についての検討 (2013)
  • Author(s): 吉澤 邦夫、北原 寛子、野口 夏代、加藤 広禄、中村 博幸、川尻 秀一
  • Organizer: 第37回 日本頭頸部癌学会
  • Place of Presentation: 京王プラザホテル（東京）
• [Presentation] 口腔扁平上皮癌の浸潤先進部のおけるCaveolin-1発現に関する免疫組織化学的検討 (2013)
  • Author(s): 加藤 広禄、木村 依世、柳瀬 瑞希、杉浦 史郎、岡宗 絢子、北原 寛子、野口 夏代、吉澤 邦夫、中村 博幸、川尻 秀一
  • Organizer: 第58回 日本口腔外科学会
  • Place of Presentation: 福岡国際会議場（福岡）
• [Presentation] AMPK 活性化薬剤による口腔扁平上皮癌の細胞増殖抑制効果の検討 (2012)
  • Author(s): 加藤広禄, 野口夏代, 吉澤邦夫, 北原寛子, 岡宗絢子, 杉浦史郎, 木村依世, 八木瑞希, 加藤阿希, 平井真理子, 川尻秀一
  • Organizer: 第57回日本口腔外科学会
  • Place of Presentation: パシフィコ横浜 (横浜)
• [Presentation] Claudin-7 expression as a prognostic factor correlates with mode of invasion in oral squamous cell carcinoma (2012)
  • Author(s): Yoshizawa K, Sugiura S, Okamune A, Kitahara H, Noguchi N, Kato K, Kawashiri S
  • Organizer: 17th World congress on advances in onclogy and 15th International symposium on molecular medicine
  • Place of Presentation: Creta Maris(Creta) , Greece
• [Presentation] Claudin-7 expression as a prognostic factor correlates with mode of invasion in ora squamous cell carcinoma (2012)
  • Author(s): Kunio Yoshizawa
  • Organizer: 17th World congress on advances in oncology and 15th International symposium on molecular medicine
  • Place of Presentation: Creta Maris Hotel （Crete-Greece）
• [Presentation] 口腔扁平上皮癌におけるClaudin7発現と浸潤様式および臨床病理学的因子との関連について (2012)
  • Author(s): Kunio Yoshizawa
  • Organizer: 第66回 NPO法人 日本口腔科学会学術集会
  • Place of Presentation: 広島国際会議場（広島市）
  • Invited