Molecular mechanism of cathechin on epidermal growth factor receptor protein degradation and growth inhibitory effect of oral cancer

• Project/Area Number: 24792196
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Surgical dentistry
• Research Institution: University of Fukui
• Principal Investigator: YOSHIMURA Hitoshi 福井大学, 医学部, 講師 (40362917)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 癌 / シグナル伝達 / 蛋白質 / タンパク質 / タンバク質

Outline of Final Research Achievements

Epidermal growth factor receptor (EGFR) is a useful molecular target in the treatment of head and neck cancer. We examined the following points.1. Evaluation of the molecular level of the utility of EGFR decomposing action by EGCG. We used the vascular smooth muscle cells that highly expressing EGFR.In our results, EGCG reduced the EGFR at the protein level by activation of ubiquitin over proteasome degradation pathway.2. Determination of tumor growth inhibitory effect of EGCG in mouse tumor xenograft model. The HSC-3 is oral squamous-cell carcinoma cell lines were transplanted into nude mice subcutaneously.Tumor volume, apoptosis, EGFR expression and phosphorylation is planned to evaluate.