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Identification of a nwe factor to promote human iPS cell generation

Research Project

Project/Area Number 24792197
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Surgical dentistry
Research InstitutionGifu University

Principal Investigator

TAMAOKI Naritaka  岐阜大学, 医学(系)研究科(研究院), 助教 (40585303)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords歯髄細胞 / iPS細胞 / 誘導効率 / DLX4 / TGF-b / 歯髄細胞(DPC) / Dlx4
Outline of Final Research Achievements

In this study, we showed that the reprogramming potential of human dental pulp cells (DPCs) obtained from immature teeth is much higher than those of mature teeth DPCs. Furthermore, immature teeth DPCs can be reprogrammed by OCT3/4 and SOX2, conversely these two factors are insufficient to convert mature teeth DPCs to pluripotent states. Using a gene expression profiles between these two DPC groups, we identified a new transcript factor, distal-less homeobox 4 (DLX4), which was highly expressed in immature teeth DPCs and significantly promoted human iPSC generation in combination with OCT3/4, SOX2, and KLF4. We further show that activation of TGF-b signaling suppresses the expression of DLX4 in DPCs and impairs the iPSC generation of DPCs. Our findings indicate that DLX4 can functionally replace c-MYC and supports efficient reprogramming of immature teeth DPCs.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (5 results)

All 2014 2013

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (3 results) Patent(Industrial Property Rights) (1 results) (of which Overseas: 1 results)

  • [Journal Article] The homeobox gene DLX4 promotes generation of human induced pluripotent stem cells2014

    • Author(s)
      Naritaka Tamaoki, Kazutoshi Takahashi, Hitomi Aoki, Kazuki Iida, Tomoko Kawaguchi, Daijirou Hatakeyama, Masatoshi Inden, Naoyuki Chosa, Akira Ishisaki, Takahiro Kunisada, Toshiyuki Shibata, Naoki Goshima, Shinya Yamanaka, and Ken-ichi Tezuka
    • Journal Title

      Scientific Reports

      Volume: なし Issue: 1 Pages: 7283-7283

    • DOI

      10.1038/srep07283

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] The homeobox gene DLX4 promotes generation of human induced pluripotent stem cells2014

    • Author(s)
      Naritaka Tamaoki, Kazutoshi Takahashi, Hitomi Aoki, Kazuki Iida, Tomoko Kawaguchi, Daijirou Hatakeyama, Masatoshi Inden, Naoyuki Chosa, Akira Ishisaki, Takahiro Kunisada, Toshiyuki Shibata, Naoki Goshima, Shinya Yamanaka, and Ken-ichi Tezuka
    • Organizer
      アメリカ骨代謝学会
    • Place of Presentation
      テキサス州、ヒューストン
    • Year and Date
      2014-09-12 – 2014-09-16
    • Related Report
      2014 Annual Research Report
  • [Presentation] ホメオボックス遺伝子DLX4はヒトiPS細胞の誘導効率を促進する2014

    • Author(s)
      ○玉置 也剛、青木 仁美、飯田 一規、川口 知子、位田 雅俊、高橋 和利、帖佐 直幸、石崎 明、國貞 隆弘、柴田 敏之、五島 直樹、手塚 建一
    • Organizer
      日本再生医療学会
    • Place of Presentation
      京都国際会議場
    • Related Report
      2013 Research-status Report
  • [Presentation] 歯髄細胞株間におけるiPS細胞への誘導効率の比較検討2013

    • Author(s)
      ○玉置也剛・川口知子・飯田一規・杉山 健・ 畠山大二郎・柴田敏之
    • Organizer
      日本口腔外科学会
    • Place of Presentation
      福岡国際会議場
    • Related Report
      2013 Research-status Report
  • [Patent(Industrial Property Rights)] 人工多能性幹細胞の作製方法2014

    • Inventor(s)
      手塚 建一、玉置 也剛、国貞 隆弘、柴田 敏之、五島 直樹
    • Industrial Property Rights Holder
      手塚 建一、玉置 也剛、国貞 隆弘、柴田 敏之、五島 直樹
    • Industrial Property Rights Type
      特許
    • Filing Date
      2014-02-26
    • Related Report
      2013 Research-status Report
    • Overseas

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Published: 2013-05-31   Modified: 2019-07-29  

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