Translational research for craniofacial region using CNP

• Project/Area Number: 24792201
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Surgical dentistry
• Research Institution: Kyoto University
• Principal Investigator: NAKAO Kazumasa 京都大学, 医学(系)研究科(研究院), 助教 (40599932)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 内軟骨骨化 / 顎顔面形態 / CNP

Research Abstract

C-type natriuretic peptide (CNP) is a potent stimulator of long bone and vertebra development via endochondral ossification. In the present study, we investigated the effects of CNP on craniofacial skeletogenesis, which consists of both endochondral and membranous ossifications. Morphometric analyses of crania from CNP knockout and transgenic mice revealed that CNP stimulates longitudinal growth along the cranium length, but does not regulate the cranium width. These results indicate that CNP primarily stimulates endochondral ossification in the craniofacial region and is crucial for midfacial skeletogenesis.

Research Products

• [Journal Article] Human induced pluripotent stem cells differentiated into chondrogenic lineage via generation of mesenchymal progenitor cells (2013)
  • Author(s): Koyama N, Miura M, Nakao K, Kondo E, Fujii T, Taura D, Kanamoto N, Sone M, Yasoda A, Arai H, Bessho K, Nakao K
  • Journal Title: Stem Cells Dev Volume: 22(1) Pages: 102-113
• [Journal Article] The Effects of C-type Natriuretic Peptide on Craniofacial Skeletogenesis (2013)
  • Author(s): Nakao K, Okubo Y, Yasoda A, Koyama N, Osawa K, Isobe Y, Kondo E, Fujii T, Miura M, Nakao K, Bessho K
  • Journal Title: J Dent Res Volume: 92(1) Pages: 58-64
• [Journal Article] The effects of C-type natriuretic peptide on craniofacial skeletogenesis (2013)
  • Author(s): Nakao K, Okubo Y, Yasoda A, Koyama N, Osawa K, Isobe Y, Kondo E, Fujii T, Miura M, Nakao K, Bessho K
  • Journal Title: J Dent Res Volume: 92(1) Issue: 1 Pages: 58-64
  • DOI: 10.1177/0022034512466413
  • Peer Reviewed
• [Presentation] 高圧酸素療法と反復腐骨少量除去術が奏功したビスフォスフォネート関連顎骨骨髄炎の 1 例 (2013)
  • Author(s): 吉岡洋祐, 中尾一祐, 斉藤和幸, 山崎亨, 高橋 克, 別所和久
  • Organizer: 第25回日本口腔科学会近畿地方部会
  • Place of Presentation: 大阪市
  • Year and Date: 2013-12-07
• [Presentation] 内軟骨性骨化の顎顔面形態に対する影響(軟骨細胞特異的 CNP ノックアウトマウスを用いた研究) (2013)
  • Author(s): 山中茂樹, 中尾一祐, 磯部悠, 池野正幸, 小山典昭, 別所和久
  • Organizer: 第25回日本口腔科学会近畿地方部会
  • Place of Presentation: 大阪市
  • Year and Date: 2013-12-07
• [Presentation] C-type natriuretic peptide (CNP) / guanyl cyclase-B (GC-B) system in growth plate promotes endochondral bone formation through autocrine/paracrine mechanism (2013)
  • Author(s): Nakao K, Yasoda A, Osawa K, Koyama N, Miura M, Akiyama H, BesshoK, Nakao K
  • Organizer: 2nd Joint Meeting of the International Bone and Mineral Society and The Japanese Society for Bone and Mineral Research approaches (IBMS2013)
  • Place of Presentation: Kobe
• [Presentation] The development of culture method for chondrogenic differentiation of human iPS cells (2013)
  • Author(s): Koyama N, Miura M, Nakao K, Kondo E, Fujii T, Kanamoto N, Yasoda A, Arai H, Bessho K, Nakao K
  • Organizer: 2nd Joint Meeting of the IBMS and the JSBMR
  • Place of Presentation: Kobe
• [Presentation] The development of culture method for chondrogenic differentiation of human iPS cells (2013)
  • Author(s): Koyama N, Miura M, Nakao K, Kondo E, Fujii T, Kanamoto N, Yasoda A, Arai H, Bessho K, Nakao K
  • Organizer: 2nd Joint Meeting of the IBMS and the JSBMR, Young Investigator Seminar
  • Place of Presentation: Kobe
• [Presentation] Lessons from tissue-specific KO of CNP/GC-B system -everlasting challenge to clinical application (2013)
  • Author(s): Nakao K, Kuwahara K, Yasoda A, Katuura G, Nakao K, Yamasita Y
  • Organizer: 6th International Conference on cGMP
  • Place of Presentation: Erfurt
• [Presentation] Impact of local CNP/GC-B system in growth plates on enchodral bone growth (2013)
  • Author(s): Nakao K, Yasoda A, Osawa K, Fujii T. Kondo E, Koyama N, Kanamoto N, Miura M, Kuwahara K, Akiyama H, BesshoK, Nakao K
  • Organizer: 6th International Conference on cGMP
  • Place of Presentation: Erfurt
• [Presentation] 内軟骨骨化における CNP/GC-B 系の生理的意義 (2013)
  • Author(s): 中尾一祐, 八十田明宏, 別所和久, 中尾一和
  • Organizer: 第19回研究発表(TMFC)成人病の病因・病態の解明に関する研究助成
  • Place of Presentation: セントレジスホテル大阪
• [Presentation] 顎顔面形態における内軟骨骨化の影響 (2013)
  • Author(s): 中尾一祐, 大久保康則, 山中茂樹, 磯部悠, 池野正幸, 小山典昭 大澤賢次, 別所和久
  • Organizer: 第58回日本口腔外科学会総会
  • Place of Presentation: 福岡市
• [Presentation] ヒト iPS 細胞を用いて間葉系前駆細胞を介した骨芽細胞への分化誘導 (2013)
  • Author(s): 磯部悠, 小山典昭, 池野正幸, 中尾一祐, 高橋克, 別所和久
  • Organizer: 第58回日本口腔外科学会総会
  • Place of Presentation: 福岡市
• [Presentation] C型ナトリウム利尿ペプチド（CNP）の顎顔面形態における影響および顎変形症に対する臨床応用の可能性 －遺伝子改変マウスを用いた解析－ (2013)
  • Author(s):  中尾一祐 八十田明宏 小山典昭 近藤絵里 藤井寿人 別所和久 中尾一和
  • Organizer: 第86回日本内分泌学会学術総会
  • Place of Presentation: 仙台
• [Presentation] Impact of local CNP/GC-B system in growth plates on enchodral bone growth. (2013)
  • Author(s): Nakao K, Yasoda A, Osawa K, Fujii T. Kondo E, Koyama N, Kanamoto N, Miura M, Kuwahara K, Akiyama H, BesshoK, Nakao K.
  • Organizer: 6th International Conference on cGMP.
  • Place of Presentation: Erfurt
• [Presentation] 顎顔面形態における内軟骨骨化の影響 (2013)
  • Author(s): 中尾一祐 大久保康則 山中茂樹 磯部悠 池野正幸 小山典昭 大澤賢次 別所和久
  • Organizer: 第58回日本口腔外科学会総会
  • Place of Presentation: 福岡
• [Presentation] 内軟骨性骨化におけるCNP/GC-B系の生理的意義 －軟骨特異的ノックアウトマウスの解析－ (2012)
  • Author(s):  中尾一祐 八十田明宏 大澤賢次 小山典昭 近藤絵里 藤井寿人 金本巨哲 三浦晶子 曽根正勝 荒井宏司 別所和久 中尾一和
  • Organizer: 第85回日本内分泌学会学術総会
  • Place of Presentation: 名古屋市
• [Presentation] C型ナトリウム利尿ペプチド(CNP)の顎顔面形態における影響および臨床応用の可能性 (2012)
  • Author(s): 中尾一祐 大久保康則 小山典昭 大澤賢次 磯部悠 別所和久
  • Organizer: 第57回日本口腔外科学会総会
  • Place of Presentation: 横浜市
• [Presentation] 内軟骨性骨化の顎顔面形態に対する影響（軟骨細胞特異的CNPノックアウトマウスを用いた研究）
  • Author(s): 山中茂樹 中尾一祐 磯部悠 池野正幸 小山典昭 別所和久
  • Organizer: 第25回日本口腔科学会近畿地方部会
  • Place of Presentation: 大阪