Epigenetic reprogramming of oral squamous cell cancer cells with oocyte extracts

Research Project

Project/Area Number	24792217
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Surgical dentistry
Research Institution	Hiroshima University
Principal Investigator	FUKUI Yasuto 広島大学, 医歯薬保健学研究院, 助教 (90363085)
Project Period (FY)	2012-04-01 – 2014-03-31
Project Status	Completed (Fiscal Year 2013)
Budget Amount *help	¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000) Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000) Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords	歯学 / リプログラミング / 卵母細胞
Research Abstract	I examined the expression of tumour suppressor gene (E-cadherin,p16,MGMT,DAP-K,MLH1) in oral squamous cell carcinoma cell line ZA using RT-PCR. MGMT,DAP-K and MLH1 were not expressed in ZA. ZA cells were added to Xenopus oocyte extracts (5,000 cells/ul extract) and incubated for 3,6,9 hours. Cells were collected and processed for RNA extraction. I examined the expression of tumour suppressor gene (MGMT,DAP-K,MLH1) in treated cells using RT-PCR. However, I did not recognize the significant difference.

Report

(3 results)