| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
|---|
| Outline of Final Research Achievements |
We demonstrate that the in vivo administration of decitabine to the murine aging model mice. Western blot analysis demonstrated the augmented expression of AQP5 protein in the salivary glands of decitabine-treated mice compared to those of control mice. In addition, AQP5 protein expression levels in 5-Aza-CdR-treated old mice (27 weeks old) were much higher than those in untreated and young mice (6 weeks old). Global methylation levels in the salivary glands were significantly lower in the decitabine-treated mice than in the untreated mice. Moreover, the induction of demethylation in the AQP5 promoter of decitabine-treated mice was stronger than in the control mice. Our data therefore suggest that a DNA demethylating agent may be a useful drug for restoring hyposalivation in elderly individuals.
|
