Establishment of sentinel node biopsy for oral cancer using one-step nucleic acid amplification method

• Project/Area Number: 24792227
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Surgical dentistry
• Research Institution: Ehime University
• Principal Investigator: GODA Hiroyuki 愛媛大学, 医学部附属病院, 助教 (00464371)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 口腔癌 / 分子診断 / OSNA 法 / リンパ節転移 / 遺伝子診断 / 口腔扁平上皮癌 / センチネルリンパ節生検

Research Abstract

We carried out a clinical study to evaluate a novel automated assay system for cytokeratin 19 (CK19) mRNA, the one-step nucleic acid amplification (OSNA) method, to detect the lymph node metastasis of OSCC. The accuracy of OSNA assay was nearly 95%, but it could not detect occult metastasis in case of no or low expression of CK19 mRNA in the primary tumor. We identified ANXA8L2 that were commonly expressed in all OSCC metastatic lymph nodes but not in benign lymph nodes. This molecular marker and CK19 detected all histopathological metastasis-positive lymph nodes in OSCC. We confirmed the expression of ANXA8L2 mRNA in all OSCC primary tumor tissues. ANXA8L2 and CK19 could be useful molecular markers for detecting lymph node metastasis of OSCC.

Research Products

• [Journal Article] One-step nucleic acid amplification for detecting lymph node metastasis of head and neck squamous cell caricinoma (2012)
  • Author(s): Goda H, Nakashiro K, Oka R, Tanaka H, Wakisaka H, Hato N, Hyodo M, Hamakawa H
  • Journal Title: Oral Oncology Volume: 48(10) Pages: 958-63
  • Peer Reviewed
• [Journal Article] One-step nucleic acid amplification for detecting lymph node metastasis of head and neck squamous cell carcinoma. (2012)
  • Author(s): Goda H, Nakashiro K, Oka R, Tanaka H, Wakisaka H, Hato N, Hyodo M, Hamakawa H.
  • Journal Title: Oral Oncology Volume: 48(10) Pages: 958-63
  • Peer Reviewed
• [Presentation] 新規分子マーカー ANXA8L2による RT-LAMP 法を用いた口腔扁平上皮癌リンパ節転移迅速診断 (2014)
  • Author(s): 岡亮太, 中城公一, 合田啓之, 岩本和樹, 浜川裕之
  • Organizer: 第32回日本口腔腫瘍学会総会
  • Place of Presentation: 札幌
  • Year and Date: 2014-01-23
• [Presentation] 多施設共同研究による口腔扁平上皮癌頸部リンパ節転移診断への OSNA 法応用の検討 (2013)
  • Author(s): 岡亮太, 中城公一, 合田啓之, 岩本和樹, 川又均, 井川香織, 北村直也, 原田耕志, 内田大亮, 今井裕, 迫田隅男, 山本哲也, 上山吉哉, 宮本洋二, 浜川裕之
  • Organizer: 第67回日本口腔科学会総会
  • Place of Presentation: 宇都宮
  • Year and Date: 2013-05-23
• [Presentation] 口腔癌の SNNS のエビデンスと今後の展望 (2012)
  • Author(s): 合田啓之, 中城公一, 住田知樹, 日野聡史, 岡亮太, 浜川裕之
  • Organizer: SNNS 研究会学術大会
  • Place of Presentation: ウインク愛知
  • Invited
• [Presentation] Sentinel lymph node biopsy for clinically N0 oral squamous cell carcinoma (2012)
  • Author(s): Goda H, Nakashiro K, Oka R, Shintani S, Hamakawa H
  • Organizer: 2012 American Society of Clinical Oncology annual meetin
  • Place of Presentation: Chicago, USA
• [Presentation] Sentinel lymph node biopsy for clinically N0 oral squamous cell carcinoma. (2012)
  • Author(s): Hiroyuki Goda, Koh-ichi Nakashiro, Ryota Oka, Satoru Shintani, Hiroyuki Hamakawa.
  • Organizer: 2012 American Society of Clinical Oncology annual meeting
  • Place of Presentation: Chicago, USA
• [Presentation] 多施設共同による口腔扁平上皮癌頸部リンパ節転移診断への OSNA 法応用の検討
  • Author(s): 岡 亮太、中城公一、合田啓之、岩本和樹、川又 均、井川加織、北村直也、原田耕志、内田大亮、今井 裕、迫田隅男、山本哲也、上山吉哉、宮本洋二、浜川裕之
  • Organizer: 第 67 回日本口腔科学会総会
  • Place of Presentation: 宇都宮
• [Presentation] 新規分子マーカー ANXA8L2 による RT-LAMP 法を用いた口腔扁平上皮癌リンパ節転移迅速診断
  • Author(s): 岡 亮太、中城公一、合田啓之、岩本和樹、浜川裕之
  • Organizer: 第 32 回 日本口腔腫瘍学会総会・学術大会
  • Place of Presentation: 札幌