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The role of Foxo1 transcription factor in the regulation of vascular formation

Research Project

Project/Area Number 24792237
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Surgical dentistry
Research InstitutionKumamoto University

Principal Investigator

TAMURA Kiyomi  熊本大学, 発生医学研究所, 助教 (90399973)

Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords血管 / Foxo1 / 胚性幹細胞(ES細胞) / 転写因子 / 胚性幹細胞
Research Abstract

Transcription factor Forkhead box O1 (Foxo1) (-/-) mice fail to develop vascular structure and are embryonic lethal by E11. So, Foxo1 is essential for the vascular development. However the mechanism is unclear.
In vitro vessel-like structure formation of ES cells are the useful examination system for vascular formation. In this model, vascular endothelial cells (ECs) derived from wild type ES cells showed the long and thin elongated morphology, but Foxo1 (-/-) EC failed to elongate. And, wild type pericytes can associate with wild type EC. Foxo1 (-/-) cells show no-association between EC and pericytes. To identify the Foxo1 target genes that are responsible for the regulation of EC elongation, I analyzed gene expression profiles of ECs and rescue studies of putative target genes. As a result, I identified an putative target gene. Furthermore, analysis of EC specific Foxo1 expressing ES cells show that Foxo1 in EC regulates EC-pericyte association.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (19 results)

All 2014 2013 2012

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (15 results) Book (3 results)

  • [Journal Article] Molecular pathways governing development of vascular endothelial cells from ES/ iPS cells.2013

    • Author(s)
      Tan KS, Tamura K, Lai MI, Veerakumarasivam A, Nakanishi Y, Ogawa M and Sugiyama D.
    • Journal Title

      Stem Cell Rev.

      Volume: 9(5) Issue: 5 Pages: 586-98

    • DOI

      10.1007/s12015-013-9450-7

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Presentation] Regulation of vascular morphogenesis by Foxo1 transcription factor via phospho-myosin light chain2014

    • Author(s)
      田村-辻 潔美, 坂本 比呂志, 小川 峰太郎
    • Organizer
      第2回 Inflammation and Regeneration Meeting
    • Place of Presentation
      品川プリンスホテル, 東京
    • Related Report
      2013 Final Research Report
  • [Presentation] Regulation of vascular morphogenesis by Foxo1 transcription factor via phospho-myosin light chain2014

    • Author(s)
      田村-辻 潔美, 坂本 比呂志, 小川 峰太郎
    • Organizer
      第2回 Inflammation and Regeneration Meeting
    • Place of Presentation
      東京, 品川プリンスホテル
    • Related Report
      2013 Annual Research Report
  • [Presentation] 休眠状態の血液幹細胞は, 転写因子 c-myb の発現レベルを低く保っている2013

    • Author(s)
      Hiroshi Sakamoto1, Naoki Takeda, Kiyomi Tsuji-Tamura, Saeka Hirota, Atsushi Hashiguchi, Tazir Ahmed and Minetaro Ogawa
    • Organizer
      第75回日本血液学会学術集会
    • Place of Presentation
      ロイトン札幌・さっぽろ芸文館・札幌市教育文化会館, 札幌
    • Related Report
      2013 Final Research Report
  • [Presentation] 転写因子Foxo1による血管内皮細胞の形態制御メカニズムの解析2013

    • Author(s)
      田村-辻 潔美, 坂本 比呂志, 小川 峰太郎
    • Organizer
      第34回日本炎症・再生医学会
    • Place of Presentation
      国立京都国際会館, 京都
    • Related Report
      2013 Final Research Report
  • [Presentation] 眠状態の血液幹細胞は、転写因子c-mybの発現レベルを低く保っている2013

    • Author(s)
      Hiroshi Sakamoto1, Naoki Takeda, Kiyomi Tsuji-Tamura, Saeka Hirota, Atsushi Hashiguchi, Tazir Ahmed and Minetaro Ogawa
    • Organizer
      第75回日本血液学会学術集会
    • Place of Presentation
      札幌, ロイトン札幌・さっぽろ芸文館・札幌市教育文化会館
    • Related Report
      2013 Annual Research Report
  • [Presentation] 転写因子Foxo1による血管内皮細胞の形態制御メカニズムの解析2013

    • Author(s)
      田村-辻 潔美、坂本 比呂志、小川 峰太郎
    • Organizer
      第34回 日本炎症・再生医学会
    • Place of Presentation
      京都, 国立京都国際会館
    • Related Report
      2013 Annual Research Report
  • [Presentation] Forkhead transcriptional factor 1 (Foxo1) regulates vascular endothelial cell morphology2013

    • Author(s)
      田村清美
    • Organizer
      有馬5拠点ミーティング
    • Place of Presentation
      有馬兵衛向陽閣(有馬)
    • Related Report
      2012 Research-status Report
  • [Presentation] Levels of the c-Myb Protein Indicate Repopulating Capacity in Long-Term Hematopoietic Stem Cells2012

    • Author(s)
      Hiroshi Sakamoto, Naoki Takeda, Kiyomi Tsuji-Tamura, Saeka Hirota and Ogawa Minetaro
    • Organizer
      2012 ASH Annual Meeting and Exposition
    • Place of Presentation
      Atlanta, GA, USA
    • Related Report
      2013 Final Research Report
  • [Presentation] Expression and function of c-Myb in hematopoietic stem cells2012

    • Author(s)
      Hiroshi Sakamoto, Naoki Takeda, Paloma Garcia, Kiyomi Tsuji-Tamura, Saeka Hirota, Jon Frampton and Minetaro Ogawa
    • Organizer
      第74回日本血液学会学術集会
    • Place of Presentation
      国立京都国際会館, 京都
    • Related Report
      2013 Final Research Report
  • [Presentation] 血管内皮細胞の形態制御に関与する転写因子Foxo1の標的因子の探索2012

    • Author(s)
      田村-辻 潔美, 坂本 比呂志, 小川 峰太郎
    • Organizer
      第33回日本炎症・再生医学
    • Place of Presentation
      ホテル日航福岡, 福岡
    • Related Report
      2013 Final Research Report
  • [Presentation] Expression and function of c-Myb in hematopoietic stem cells2012

    • Author(s)
      Hiroshi Sakamoto, Takeda Naoki, Paloma Garcia, Kiyomi Tsuji-Tamura, Saeka Hirota, Jon Frampton, Minetaro Ogawa
    • Organizer
      第10回幹細胞シンポジウム
    • Place of Presentation
      淡路夢舞台国際会議場, 淡路島
    • Related Report
      2013 Final Research Report
  • [Presentation] Levels of the c-Myb Protein Indicate Repopulating Capacity in Long-Term Hematopoietic Stem Cells2012

    • Author(s)
      Hiroshi Sakamoto, Naoki Takeda, Kiyomi Tsuji-Tamura, Saeka Hirota1 and Ogawa Minetaro
    • Organizer
      2012 ASH (American society of hematology) Annual Meeting and Exposition
    • Place of Presentation
      Atlanta, GA, USA
    • Related Report
      2012 Research-status Report
  • [Presentation] 転写因子c-mybの血液幹細胞における発現と機能2012

    • Author(s)
      Hiroshi Sakamoto, Naoki Takeda, Paloma Garcia, Kiyomi Tsuji-Tamura, Saeka Hirota, Jon Frampton and Minetaro Ogawa
    • Organizer
      第74回日本血液学会学術集会
    • Place of Presentation
      国立京都国際会館(京都)
    • Related Report
      2012 Research-status Report
  • [Presentation] 血管内皮細胞の形態制御に関与する転写因子Foxo1の標的因子の探索2012

    • Author(s)
      田村-辻 潔美、坂本 比呂志、小川 峰太郎
    • Organizer
      第33回日本炎症・再生医学
    • Place of Presentation
      ホテル日航福岡(福岡)
    • Related Report
      2012 Research-status Report
  • [Presentation] Expression and function of c-Myb in hematopoietic stem cells2012

    • Author(s)
      Hiroshi Sakamoto, Takeda Naoki, Paloma Garcia, Kiyomi Tsuji-Tamura, Saeka Hirota, Jon Frampton, Minetaro Ogawa
    • Organizer
      第10回幹細胞シンポジウム
    • Place of Presentation
      淡路夢舞台国際会議場(淡路島)
    • Related Report
      2012 Research-status Report
  • [Book] 微小環境の血液幹細胞への働きかけ-誕生から骨髄での維持まで, 実験医学増刊-臓器円環による生体恒常性のダイナミクス, 永井良三, 入來篤史/編2013

    • Author(s)
      坂本比呂志, 田村潔美, 小川峰太郎
    • Publisher
      羊土社
    • Related Report
      2013 Final Research Report
  • [Book] 実験医学増刊-臓器円環による生体恒常性のダイナミクス, 微小環境の血液幹細胞への働きかけ─誕生から骨髄での維持まで2013

    • Author(s)
      坂本比呂志, 田村潔美, 小川峰太郎
    • Publisher
      羊土社
    • Related Report
      2013 Annual Research Report
  • [Book] 実験医学増刊(臓器円環による生体恒常性のダイナミクス、永井良三、入來篤史/編)Vol.31 No.52013

    • Author(s)
      坂本比呂志、田村潔美、小川峰太郎
    • Publisher
      羊土社
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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