To clalify the new regulator of cell-cell fusion of osteoclasts

• Project/Area Number: 24792252
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Surgical dentistry
• Research Institution: Keio University
• Principal Investigator: RYOTARO Iwasaki 慶應義塾大学, 医学部, 講師(非常勤) (30365390)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 骨代謝 / 細胞融合 / 破骨細胞

Outline of Final Research Achievements

Osteoclasts are multinuclear giant cells derived from hematopoietic stem cells　or monocyte/macrophage lineage cells in the presence of M-CSF and RANKL. The multinucleation of osteoclasts is induced by a cell-cell fusion of mononuclear osteoclasts. Recently, we have isolated DC-STAMP (Dendritic Cell Specific Transmenbrane Protein), a seven transmembrane protein, as an essential molecule for osteoclast/FBGC cell-cell fusion.
This time, we identified the multitransmembrane protein,osteoclast stimulatory transmembrane protein(OC-STAMP), and found that OC-STAMP was also required for osteoclast and FBGC cell-cell fusion.

Research Products

• [Journal Article] TNFα promotes osteosarcoma progression by maintaining tumor cells in an undifferentiated state. (2013)
  • Author(s): Mori T,Sato Y Iwasaki R et.al.,
  • Journal Title: Oncogene Volume: 545 Pages: 1-6
  • Peer Reviewed
• [Journal Article] OC-STAMP and DC-STAMP cooperatively modulate cell-cell fusion to form osteoclasts and foreign body giant cells (2012)
  • Author(s): Miyamoto H, et al
  • Journal Title: J Bone Miner Res Volume: (In press) Issue: 6 Pages: 1289-1297
  • DOI: 10.1002/jbmr.1575
  • Peer Reviewed
• [Journal Article] PDGFBB promotes PDGFRα-positive cell migration into artificial bone in vivo. (2012)
  • Author(s): Yoshida S, Iwasaki R, Kawana H, Miyauchi Y, Hoshi H, Miyamoto H, Mori T, Kanagawa H et al.
  • Journal Title: Biochem Biophys Res Commun. Volume: 421 Issue: 4 Pages: 785-789
  • DOI: 10.1016/j.bbrc.2012.04.084
  • Peer Reviewed
• [Journal Article] Aldehyde-stress resulting from Aldh2 mutation promotes osteoporosis due to impaired osteoblastogenesis (2012)
  • Author(s): Hoshi H, Hao W, Fujita Y, Funayama A, Miyauchi Y, Hashimoto K, Miyamoto K, (他21名)
  • Journal Title: J. Bone Miner Res Volume: 27(9) Issue: 9 Pages: 2015-2023
  • DOI: 10.1002/jbmr.1634
  • Peer Reviewed
• [Presentation] 破骨細胞に発現するSmad4は箱津市安房の分化抑制から骨量を減少する (2014)
  • Author(s): 森田麻友、岩崎良太郎、宮本健史、河奈裕正、中川種昭
  • Organizer: 第５９回日本口腔外科学会総会
  • Place of Presentation: 幕張メッセ 国際会議場、東京 千葉
  • Year and Date: 2014-10-17 – 2014-10-19
• [Presentation] Both Cytokines, TNFα and IL-1 are Essential for the Tumorigenesis of Mouse Osteosarcoma. (2014)
  • Author(s): Ryotaro Iwasaki,Takeshi Miyamoto ,Mayu Morita ,Tomoaki Mori ,Taneaki Nakagawa,Hiromsa Kawana
  • Organizer: 96th AAOMS Annual Meeting, Scientific Sessions and Exhibition
  • Place of Presentation: Hawaii Convention Center、USA
  • Year and Date: 2014-09-08 – 2014-09-13
• [Presentation] TNFα and IL-1 are both essential cytokines for the tumorigenesis of mouse osteosarcoma cell line AX. (2013)
  • Author(s): Iwasaki R,Miyamoto T et.al.,
  • Organizer: 21th International conference on pral and maxillofacial surgery
  • Place of Presentation: Barcelona,Spain
• [Presentation] TNFαおよびIL-1はマウス骨肉腫細胞株AXの腫瘍形成に必須である (2013)
  • Author(s): 岩崎良太郎、宮本健史、森田麻友、臼田慎、宮下英高、莇生田整治、中川種昭、河奈裕正
  • Organizer: 第５８回日本口腔外科学会総会
  • Place of Presentation: 福岡国際会議場、福岡県
• [Presentation] PDGF-BB promotes PDGFRα-positive cell migration into artificial bone in vivo (2012)
  • Author(s): Yoshida S, Iwasaki R, Kawana H, Nakagawa T, Miyamoto T
  • Organizer: ASBMR Topical meetings bone and skeletal muscle
  • Place of Presentation: ASBMR Topical meetings bone and skeletal muscle
• [Presentation] PDGFBBは人工骨の生体親和性を上昇させる (2012)
  • Author(s): 吉田重之、岩﨑良太郎、河奈裕正、中川種昭、宮本健史
  • Organizer: 第30回 日本骨代謝学会学術集会
  • Place of Presentation: 京王プラザホテル 新宿 東京
• [Presentation] PDGF-BB promotes PDGFRα-positive cell migration into artificial bone in vivo (2012)
  • Author(s): Yoshida S, Iwasaki R, Kawana H, Nakagawa T, Miyamoto T
  • Organizer: 2012 ASBMR annual meeting
  • Place of Presentation: Minneapolis convention center at Minneapolis, Minnesota, USA
• [Presentation] PDGFR alpha-positive cells were migrated into artificial bone by PDGFBB in vivo. (2012)
  • Author(s): R. Iwasaki, S. Yoshida, T. Miyamoto, T. Nakagawa, H. Kawana
  • Organizer: 10th Asian Congress on Oral and Maxillofacial surgery
  • Place of Presentation: Discovery Kartika Plaza Hotel Bali-Indonesia