| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Outline of Final Research Achievements |
We examined whether propofol differentially regulates inhibitory postsynaptic currents (IPSCs) depending on the pre- and postsynaptic cell subtypes in rat insular cortex. Propofol (10 μM) consistently prolonged decay kinetics of unitary IPSCs (uIPSCs) in all types of inhibitory connections without changing paired-pulse ratio or failure rate. The fast-spiking (FS) to Pyr connections exhibited greater enhancement of uIPSC charge transfer compared to that of FS to FS/non-FS connections, whereas the enhancement of charge transfer in non-FS to Pyr/FS/non-FS connections was smaller to those in FS to Pyr/FS/non-FS. The principal inhibitory connections (FS to Pyr) are the most sensitive to propofol-induced facilitation of uIPSCs, which is likely mediated by postsynaptic mechanisms. This preferential uIPSC enhancement in FS to Pyr connections may result in suppressed neural activities of projection neurons, which in turn reduces excitatory outputs from cortical local circuits.
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