Pre- and post-synaptic cell type dependent modulation of GABAergic synaptic transmission by propofol in rat insular cortex
| Project/Area Number |
24792257
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Surgical dentistry
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| Research Institution | Nihon University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | プロポフォール / 抑制性シナプス伝達 / 大脳皮質 / fast-spiking細胞 / 錐体細胞 / GABA(A)受容体 / 抑制性シナプス後電流 / ホールセル・パッチクランプ法 / propofol / GABA(A) receptor / cortex / IPSC / whole-cell patch-clamp |
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| Outline of Final Research Achievements |
We examined whether propofol differentially regulates inhibitory postsynaptic currents (IPSCs) depending on the pre- and postsynaptic cell subtypes in rat insular cortex. Propofol (10 μM) consistently prolonged decay kinetics of unitary IPSCs (uIPSCs) in all types of inhibitory connections without changing paired-pulse ratio or failure rate. The fast-spiking (FS) to Pyr connections exhibited greater enhancement of uIPSC charge transfer compared to that of FS to FS/non-FS connections, whereas the enhancement of charge transfer in non-FS to Pyr/FS/non-FS connections was smaller to those in FS to Pyr/FS/non-FS. The principal inhibitory connections (FS to Pyr) are the most sensitive to propofol-induced facilitation of uIPSCs, which is likely mediated by postsynaptic mechanisms. This preferential uIPSC enhancement in FS to Pyr connections may result in suppressed neural activities of projection neurons, which in turn reduces excitatory outputs from cortical local circuits.
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Report
(4 results)
Research Products
(10 results)
