Basic research of bone tissue regeneration using human dedifferentiated fat cells for clinical application

• Project/Area Number: 24792263
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Surgical dentistry
• Research Institution: Osaka Dental University
• Principal Investigator: KISHIMOTO Naotaka 大阪歯科大学, 歯学部, 助教 (50610911)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 脱分化脂肪細胞 / 脂肪組織由来幹細胞 / 頬脂肪体 / 骨再生 / 組織工学 / 天井培養 / 脂肪幹細胞

Research Abstract

We isolated human dedifferentiated fat (DFAT) cells and adipose-derived stem cells (ASC) from the buccal fat pad, and evaluated and compared the osteoblastic differentiation ability. Bone-specific alkaline phosphatase, osteocalcin, and calcium as osteoblastic differentiation markers within DFAT cell cultures were significantly higher than those in ASC cultures. The osteoblastic differentiation ability of DFAT cells is higher than that of the ASC from the buccal fat pad. We consider that DFAT cells from the buccal fat pad are useful cell source for bone tissue engineering.

Research Products

• [Journal Article] The osteoblastic differentiation ability of human dedifferentiated fat cells is higher than that of adipose stem cells from the buccal fat pad. (2014)
  • Author(s): Kishimoto N, Momota Y, Hashimoto Y, Tatsumi S, Ando K, Omasa T, Kotani J.
  • Journal Title: Clin Oral Investig. Volume: In press Issue: 8 Pages: 1893-1901
  • DOI: 10.1007/s00784-013-1166-1
  • Peer Reviewed
• [Journal Article] The utility of human dedifferentiated fat cells in bone tissue engineering in vitro. (2014)
  • Author(s): Sakamoto F, Hashimoto Y, Kishimoto N, Honda Y, Matsumoto N.
  • Journal Title: Cytotechnology. Volume: In press Issue: 1 Pages: 75-84
  • DOI: 10.1007/s10616-013-9659-y
  • Peer Reviewed
• [Presentation] ヒト頬脂肪体由来脱分化脂肪細胞と脂肪幹細胞の細胞表面抗原解析 (2013)
  • Author(s): 岸本 直隆
  • Organizer: 第11回日本再生歯科医学会学術大会
  • Place of Presentation: 東京
  • Year and Date: 2013-08-31
• [Presentation] Comparison of Osteoblastic Differentiation Abilities in Dedifferentiated-Fat-Cells with Adipose-Stem-Cells (2013)
  • Author(s): 岸本 直隆
  • Organizer: The 91st International Association for Dental Research General Session & Exhibition
  • Place of Presentation: Seattle, Washington, USA
  • Year and Date: 2013-03-23
• [Presentation] 脱分化脂肪細胞を用いた顎骨再生におけるトランスレーショナル研究 (2013)
  • Author(s): 岸本 直隆
  • Organizer: 第29回 「歯科医学を中心とした総合的な研究を推進する集い」
  • Place of Presentation: 東京
  • Year and Date: 2013-01-12
• [Presentation] Osteoblastic Differentiation Ability is higher in Human Dedifferentiated Fat Cells than in Adipose Stem Cells (2012)
  • Author(s): 岸本 直隆
  • Organizer: YABEC2012
  • Place of Presentation: 徳島
  • Year and Date: 2012-10-27
• [Presentation] ヒト脱分化脂肪細胞は脂肪幹細胞より骨芽細胞分化能が高い (2012)
  • Author(s): 岸本 直隆
  • Organizer: 第10回日本再生歯科医学会学術大会
  • Place of Presentation: 神戸
  • Year and Date: 2012-09-02
• [Presentation] ヒト頬脂肪体由来脱分化脂肪細胞と脂肪幹細胞の細胞表面抗原解析
  • Author(s): 岸本直隆、百田義弘、橋本典也、安東佳代子、大政健史、小谷順一郎
  • Organizer: 第11回日本再生歯科医学会学術大会
  • Place of Presentation: 日本大学理工学部駿河台キャンパスCSTホール
• [Presentation] Comparison of Osteoblastic Differentiation Abilities in Dedifferentiated-Fat-Cells with Adipose-Stem-Cells.
  • Author(s): Kishimoto N, Momota Y, Hashimoto Y, Ando K, Omasa T, Kotani J
  • Organizer: 91st General Session & Exhibition of the IADR
  • Place of Presentation: SEATTLE, WASH., USA
• [Presentation] 脱分化脂肪細胞を用いた顎骨再生におけるトランスレーショナル研究
  • Author(s): 岸本 直隆, 百田 義弘, 橋本 典也, 安東 佳代子, 大政 健史, 小谷 順一郎
  • Organizer: 第29回「歯科医学を中心とした総合的な研究を推進する集い」
  • Place of Presentation: 東京