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Effect of the occlusal stimuli on osteoclastgenesis induced by periodontal fibroblasts.

Research Project

Project/Area Number 24792320
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Periodontal dentistry
Research InstitutionHokkaido University

Principal Investigator

TAKAHASHI TOMOMI  北海道大学, 歯学研究科(研究院), 技術専門職員 (50399953)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords歯根膜線維芽細胞 / 破骨細胞誘導能
Outline of Final Research Achievements

This study was aimed to investigate the effect of the occlusal stimuli on osteoclastgenesis induced by periodontal ligament (PDL) cells.PDL cells were isolated from lower molar teeth of Wistar rats which were treated with extraction of upper molar teeth in one side 3 weeks previously.In co-cultures of murine osteoclastic precursors (RAW 264.7) and PDL cells on both occlusal and non-occlusal side, the formation of multinucleated, TRAP positive cells and molecules involved in osteoclast formation were evaluated.The results showed that the number of osteoclasts and the size of cells were significantly augmented in non-occlusal side compared to occlusal side. In addition, OPG expression of PDL cells in non-occlusal side was slightly reduced on Western blotting compared to occlusal side.
These findings suggest that the occlusal stimuli on periodontal ligament strongly influence osteoclast differentiation via OPG induced by PDL cells.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (6 results)

All 2014 2013 2012

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 1 results) Presentation (3 results)

  • [Journal Article] Effect of tunnel structure of beta-TCP on periodontal repair in class III furcation defect in dogs.2014

    • Author(s)
      Akira SAITO, Emiko SAITO, Yasuo UEDA, Yoshihiro SHIBUKAWA, Yoshiyuki HONMA, Tomomi TAKAHASHI, Marioko KIMURA, Yoshinori KUBOKI, Hiroshi KATO
    • Journal Title

      Bioceramics Development and Applications

      Volume: 4(1) Issue: 1 Pages: 73-73

    • DOI

      10.4172/2090-5025.1000073

    • Related Report
      2014 Annual Research Report 2013 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Periodontal regeneration following application of basic fibroblast growth factor-2 in combination with beta tricalcium phosphate in class III furcation defects in dogs2013

    • Author(s)
      Akira SAITO, Emiko SAITO, Yoshinori KUBOKI, Marioko KIMURA, Toshinori NAKAJIMA, Funihiko YUGE, Tsuyoshi KATO, Yoshiyuki HONMA, Tomomi TAKAHASHI, Nobuo OHATA
    • Journal Title

      Dental Materials Journal

      Volume: 32 Issue: 2 Pages: 256-262

    • DOI

      10.4012/dmj.2012-171

    • NAID

      10031151409

    • ISSN
      0287-4547, 1881-1361
    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Periodontal repair following implantation of beta-tricalcium phosphate with different pore structures in class III furcation defects in dogs2012

    • Author(s)
      E.SAITO, A.SAITO, Y.KUBOKI, M.KIMURA, Y.HONMA, T.TAKAHASHI, and M.KAWANAMI
    • Journal Title

      Dental Materials Journal

      Volume: 31 Issue: 4 Pages: 681-688

    • DOI

      10.4012/dmj.2011-259

    • NAID

      10030501930

    • ISSN
      0287-4547, 1881-1361
    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] 歯根膜線維芽細胞による破骨細胞制御機能に及ぼす咬合力の影響2014

    • Author(s)
      高橋智美、牛島夏未、滝田裕子、飯塚 正
    • Organizer
      第56回歯科基礎医学会学術大会・総会
    • Place of Presentation
      福岡国際会議場(福岡県福岡市)
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] ラット歯肉由来間葉系幹細胞による硬組織形成について2013

    • Author(s)
      高橋智美、牛島夏未、滝田裕子、飯塚 正
    • Organizer
      第55回歯科基礎医学会学術大会・総会
    • Place of Presentation
      岡山コンベンションセンター(岡山市)
    • Related Report
      2013 Research-status Report
  • [Presentation] Periodontal healing following various implantation methods of BMP-2, bFGF-2 and EMD in circumferential defects.2013

    • Author(s)
      Emiko Saito, Akira Saito, Shigeru Takahashi, Fumihiko Yuge, Toshinori Nakajima, Hiroki Uekita, Yoshiyuki Honma, Tomomi Takahashi, Hiroshi Kato, Masamitsu Kawanami
    • Organizer
      The American Academy of Periodontology (AAP) 99th Annual meeting
    • Place of Presentation
      Philadelphia’s Pennsylvania Convention Center (USA)
    • Related Report
      2013 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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