Effect of the occlusal stimuli on osteoclastgenesis induced by periodontal fibroblasts.

• Project/Area Number: 24792320
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Periodontal dentistry
• Research Institution: Hokkaido University
• Principal Investigator: TAKAHASHI TOMOMI 北海道大学, 歯学研究科(研究院), 技術専門職員 (50399953)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 歯根膜線維芽細胞 / 破骨細胞誘導能

Outline of Final Research Achievements

This study was aimed to investigate the effect of the occlusal stimuli on osteoclastgenesis induced by periodontal ligament (PDL) cells.PDL cells were isolated from lower molar teeth of Wistar rats which were treated with extraction of upper molar teeth in one side 3 weeks previously.In co-cultures of murine osteoclastic precursors (RAW 264.7) and PDL cells on both occlusal and non-occlusal side, the formation of multinucleated, TRAP positive cells and molecules involved in osteoclast formation were evaluated.The results showed that the number of osteoclasts and the size of cells were significantly augmented in non-occlusal side compared to occlusal side. In addition, OPG expression of PDL cells in non-occlusal side was slightly reduced on Western blotting compared to occlusal side.
These findings suggest that the occlusal stimuli on periodontal ligament strongly influence osteoclast differentiation via OPG induced by PDL cells.

Research Products

• [Journal Article] Effect of tunnel structure of beta-TCP on periodontal repair in class III furcation defect in dogs. (2014)
  • Author(s): Akira SAITO, Emiko SAITO, Yasuo UEDA, Yoshihiro SHIBUKAWA, Yoshiyuki HONMA, Tomomi TAKAHASHI, Marioko KIMURA, Yoshinori KUBOKI, Hiroshi KATO
  • Journal Title: Bioceramics Development and Applications Volume: 4(1) Issue: 1 Pages: 73-73
  • DOI: 10.4172/2090-5025.1000073
  • Peer Reviewed / Open Access
• [Journal Article] Periodontal regeneration following application of basic fibroblast growth factor-2 in combination with beta tricalcium phosphate in class III furcation defects in dogs (2013)
  • Author(s): Akira SAITO, Emiko SAITO, Yoshinori KUBOKI, Marioko KIMURA, Toshinori NAKAJIMA, Funihiko YUGE, Tsuyoshi KATO, Yoshiyuki HONMA, Tomomi TAKAHASHI, Nobuo OHATA
  • Journal Title: Dental Materials Journal Volume: 32 Issue: 2 Pages: 256-262
  • DOI: 10.4012/dmj.2012-171
  • NAID: 10031151409
  • ISSN: 0287-4547, 1881-1361
  • Peer Reviewed
• [Journal Article] Periodontal repair following implantation of beta-tricalcium phosphate with different pore structures in class III furcation defects in dogs (2012)
  • Author(s): E.SAITO, A.SAITO, Y.KUBOKI, M.KIMURA, Y.HONMA, T.TAKAHASHI, and M.KAWANAMI
  • Journal Title: Dental Materials Journal Volume: 31 Issue: 4 Pages: 681-688
  • DOI: 10.4012/dmj.2011-259
  • NAID: 10030501930
  • ISSN: 0287-4547, 1881-1361
  • Peer Reviewed
• [Presentation] 歯根膜線維芽細胞による破骨細胞制御機能に及ぼす咬合力の影響 (2014)
  • Author(s): 高橋智美、牛島夏未、滝田裕子、飯塚 正
  • Organizer: 第５６回歯科基礎医学会学術大会・総会
  • Place of Presentation: 福岡国際会議場（福岡県福岡市）
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] ラット歯肉由来間葉系幹細胞による硬組織形成について (2013)
  • Author(s): 高橋智美、牛島夏未、滝田裕子、飯塚 正
  • Organizer: 第５５回歯科基礎医学会学術大会・総会
  • Place of Presentation: 岡山コンベンションセンター（岡山市）
• [Presentation] Periodontal healing following various implantation methods of BMP-2, bFGF-2 and EMD in circumferential defects. (2013)
  • Author(s): Emiko Saito, Akira Saito, Shigeru Takahashi, Fumihiko Yuge, Toshinori Nakajima, Hiroki Uekita, Yoshiyuki Honma, Tomomi Takahashi, Hiroshi Kato, Masamitsu Kawanami
  • Organizer: The American Academy of Periodontology (AAP) 99th Annual meeting
  • Place of Presentation: Philadelphia’s Pennsylvania Convention Center (USA)