Elucidation of the mechanism of BDNF-induced suppression of gingival epithelium invasion in the process of periodontal tissue regeneration

• Project/Area Number: 24792330
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Periodontal dentistry
• Research Institution: Hiroshima University
• Principal Investigator: MATSUDA Shinji 広島大学, 大学病院, 病院助教 (30611321)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: BDNF / 歯周組織再生 / 歯肉上皮細胞 / アポトーシス

Research Abstract

Brain-derived neurotrophic factor (BDNF) did not affect proliferation of human gingival epithelial cells (HGEC) while BDNF enhanced the proliferation of human periodontal ligament cells (HPL cells). Furthermore BDNF at high concentration induced apoptosis of HGEC. BDNF-induced apoptosis was activated by caspase-3 through p75 receptor and JNK signaling pathway. These findings suggested that BDNF can activate not trkB-ERK but p75-JNK signaling cascade, which do not affect gingival epithelial cells proliferation.

Research Products

• [Journal Article] BDNF mimetic compound LM22A-4 regulates cementoblast differentiation via the TrkB-ERK/Akt signaling cascade (2014)
  • Author(s): Kajiya M, Takeshita K, Kittaka M, Matsuda S, Ouhara K, Takeda K, Takata T, Kitagawa M, Fujita T, Shiba H, Kurihara H
  • Journal Title: Int Immunopharmacol Volume: 19(2) Pages: 245-52
  • Peer Reviewed
• [Journal Article] BDNF mimetic compound LM22A-4 regulates cementoblast differentiation via the TrkB-ERK/Akt signaling cascade. (2014)
  • Author(s): Kajiya M, Takeshita K, Kittaka M, Matsuda S, Ouhara K, Takeda K, Takata T, Kitagawa M, Fujita T, Shiba H, Kurihara H
  • Journal Title: International Immunopharmacology Volume: 19 Pages: 245-252
  • Peer Reviewed
• [Journal Article] Irsogladine maleate regulates the inflammatory related genes in human gingival epithelial cells stimulated by Aggregatibacter actinomycetemcomitans (2013)
  • Author(s): Miyagawa T, Fujita T, Ouhara K, Matsuda S, Kajiya M, Hayashida K, Imai H, Yoshimoto T, Iwata T, Shiba H, Abiko Y, Kurihara H
  • Journal Title: Int Immunopharmacol Volume: 15(2) Pages: 340-7
  • Peer Reviewed
• [Journal Article] Irsogladine maleate regulates the inflammatory related genes in human gingival epithelial cells stimulated by Aggregatibacter actinomycetemcomitans. (2013)
  • Author(s): Miyagawa T, Fujita T, Ouhara K, Matsuda S, Kajiya M, Hayashida K, Imai H, Yoshimoto T, Iwata T, Shiba H, Abiko Y, Kurihara H.
  • Journal Title: International Immunopharmacology Volume: 15 Pages: 340-347
  • Peer Reviewed
• [Journal Article] Irsogladine maleate regulates the inflammatory related genes in human gingival epithelial cells stimulated by Aggregatibacter actinomycetemcomitans. (2013)
  • Author(s): Miyagawa T
  • Journal Title: Int Immunopharmacol Volume: 15 Issue: 2 Pages: 340-347
  • DOI: 10.1016/j.intimp.2012.12.011
  • Peer Reviewed
• [Presentation] 脳由来神経栄養因子はヒト歯肉上皮細胞のアポトーシスカスケードを活性化する (2013)
  • Author(s): 柏井桂, 加治屋幹人, 藤田剛, 松田真司, 武田克浩, 柴秀樹, 栗原英見
  • Organizer: 日本歯科保存学会 2013年度秋季学術大会(第139回)
  • Place of Presentation: 秋田
• [Presentation] 脳由来神経栄養因子はヒト歯肉上皮細胞のアポトーシスカスケードを活性化する (2013)
  • Author(s): 柏井 桂
  • Organizer: 日本歯科保存学会2013年度秋季学術大会(第139回)
  • Place of Presentation: 秋田
• [Presentation] 脳由来神経栄養因子のヒト歯肉上皮細胞に与える影響 (2012)
  • Author(s): 柏井桂, 加治屋幹人, 藤田剛, 松田真司, 武田克浩, 柴秀樹, 栗原英見
  • Organizer: 第137回日本歯科保存学会学術大会(秋季)
  • Place of Presentation: 広島
• [Presentation] The effect of BDNF on human gingival epithelial cells (2012)
  • Author(s): K.Kashiwai, S.Matsuda, T.Fujita, M.Kajiya, K.Takeda, H.Shiba, H.Kurihara
  • Organizer: The American Academy of Periodontology
  • Place of Presentation: ロサンゼルス, アメリカ
• [Presentation] 脳由来神経栄養因子のヒト歯肉上皮細胞に与える影響 (2012)
  • Author(s): 柏井桂
  • Organizer: 第137回日本歯科保存学会
  • Place of Presentation: 広島