Basic study aimed at the control of insulin resistance induced periodontal inflammation that target miRNA

Research Project

Project/Area Number	24792331
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Periodontal dentistry
Research Institution	Hiroshima University
Principal Investigator	YAMASHITA Akiko 広島大学, 医歯薬保健学研究院, 助教 (70511319)
Project Period (FY)	2012-04-01 – 2014-03-31
Project Status	Completed (Fiscal Year 2013)
Budget Amount *help	¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000) Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000) Fiscal Year 2012: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywords	歯周病 / メタボリックシンドローム
Research Abstract	RAW264.7, a murine macrophage cell line and differentiated 3T3-L1 adipocytes were co-cultured. I analyzed miRNA differentially expressed in adipocytes when co-cultured with macrophages in the presenceof LPS. miRNA differentially expressed in adipocytes were analyzed by the microarray method following 4,8,12 and 24h stimulation with LPS. I compared these results with previously reports. (Yamashita A.et al., Int J Obese.,2008),extracted the data of revelant miRNA about vascular involvement and insulin resistance. The revalant miRNA about inflammation, metabolism and vascular involvement expression were significantly up and down regulated.