| Project/Area Number |
24792332
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Periodontal dentistry
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| Research Institution | The University of Tokushima |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 歯周病 / 単球 / LPS / TNF-α / TDP-43 / 転写 |
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| Research Abstract |
In previous study, TDP-43 was identified as a candidate of novel transcription factor for TNF-alpha gene regulation in periodontitis. The aim of this study was to investigated a role of TDP-43 in human monocytes/macrophages. TDP-43 localized constitutively in nuclei of differentiated THP-1 cells. LPS increased TDP-43 expression in nuclei of differentiated-THP-1 cells immediately. Overexpression of TDP-43 in THP-1 cells resulted in an increase of TNF-alpha gene expression. Inversely, knockdown of TDP-43 in THP-1 cells downregulated LPS-induced TNF-alpha gene expression. Moreover, it was suggested that the target region located -550 to -487 in TNF-alpha promoter bound several nuclear proteins such as TDP-43 and p65. These results have shown that TDP-43 may play a role as an up-regulator of LPS-induced TNF-alpha gene expression by acting in combination with other factors such as NF-kappaB in nuclei of monocytes and macrophages.
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