A role of TDP-43, a candidate of novel transcription factor for TNF-alpha gene regulation, in the pathogenesis of periodontitis.
Project/Area Number |
24792332
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Periodontal dentistry
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Research Institution | The University of Tokushima |
Principal Investigator |
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Project Period (FY) |
2012-04-01 – 2014-03-31
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Project Status |
Completed (Fiscal Year 2013)
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Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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Keywords | 歯周病 / 単球 / LPS / TNF-α / TDP-43 / 転写 |
Research Abstract |
In previous study, TDP-43 was identified as a candidate of novel transcription factor for TNF-alpha gene regulation in periodontitis. The aim of this study was to investigated a role of TDP-43 in human monocytes/macrophages. TDP-43 localized constitutively in nuclei of differentiated THP-1 cells. LPS increased TDP-43 expression in nuclei of differentiated-THP-1 cells immediately. Overexpression of TDP-43 in THP-1 cells resulted in an increase of TNF-alpha gene expression. Inversely, knockdown of TDP-43 in THP-1 cells downregulated LPS-induced TNF-alpha gene expression. Moreover, it was suggested that the target region located -550 to -487 in TNF-alpha promoter bound several nuclear proteins such as TDP-43 and p65. These results have shown that TDP-43 may play a role as an up-regulator of LPS-induced TNF-alpha gene expression by acting in combination with other factors such as NF-kappaB in nuclei of monocytes and macrophages.
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Report
(3 results)
Research Products
(13 results)
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[Presentation] 糖尿病関連歯周炎の診断マーカーとしての歯肉溝滲出液中グリコアルブミンとカルプロテクチンの有用性2013
Author(s)
木戸淳一, 板東美香, 坂東由紀子, 稲垣 裕司, 廣島佑香, 梶浦由加里, 美原智恵, 村田裕美, 生田貴久, 篠原宏貴, 橋本万里, 舟木真理, 斉藤晴比呂, 永田俊彦
Organizer
第56回日本糖尿病学会
Place of Presentation
ホテル日航熊本(熊本県)
Year and Date
2013-05-17
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