Inhibition of Spry2 Induces Proliferation and Differentiation of Osteoblasts through bFGF and EGF Stimulation but Inhibits Proliferation of Gingival Epithelial Cells.
| Project/Area Number |
24792334
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Periodontal dentistry
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| Research Institution | Kyushu University |
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Principal Investigator |
SANUI Terukazu 九州大学, 歯学研究科(研究院), 助教 (70507780)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | Spry2 / 歯周組織再生 / ｂFGF / EGF / 骨芽細胞 / 歯肉上皮細胞 / bFGF / Sprouty2 / 上皮細胞 |
|---|
| Research Abstract |
Transduction of a dominant-negative mutant of Spry2 (Y55A-Spry2) enhanced basic fibroblast growth factor (bFGF)- and epidermal growth factor (EGF)-induced ERK activation in MC3T3-E1 osteoblastic cells. In contrast, it decreased their activation in GE1 cells. Consistent with these observations, Y55A-Spry2 increased osteoblast proliferation with bFGF and EGF stimulation, whereas the proliferation of Y55A-Spry2-introduced GE1 cells was decreased via the ubiquitination and degradation of EGF receptors (EGFRs). In addition, Y55A-Spry2 caused upregulation of Runx2 expression and downregulation of Twist, a negative regulator of Runx2, with treatment of bFGF and EGF, resulting in enhanced osteoblastogenesis accompanied by alkaline phosphatase activation and osteocalcin expression in MC3T3-E1 cells.
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Report
(3 results)
Research Products
(50 results)
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[Presentation] Proteome analysis of amelogenin-binding proteins.2012
Author(s)
Urara Tanaka, Takao Fukuda, Terukazu Sanui, Kyosuke Toyoda, Takaharu Taketomi, Ryo Atomura, Takafumi Hamachi and Katsumasa Maeda
Organizer
Pan Europe Region / International Association for Dental Research (PER/IADR）Meeting
Place of Presentation
Helsinki, Finland
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[Book] 歯界展望 特別号20132013
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讃井 彰一, 田中麗, 豊田敬介, 福田隆男, 後村亮, 濱地 貴文, 前田 勝正
Total Pages
1
Publisher
医歯薬出版株式会社
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[Book] 歯界展望 特別号20132013
Author(s)
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Publisher
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[Book] 歯界展望 特別号20132013
Author(s)
田中麗, 豊田敬介, 福田隆男, 讃井 彰一, 後村亮, 濱地 貴文, 前田 勝正
Total Pages
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Publisher
医歯薬出版株式会社
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