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Dental calculus induces IL-1 beta production through NLRP3 inflammasome

Research Project

Project/Area Number 24792335
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Periodontal dentistry
Research InstitutionNagasaki University

Principal Investigator

NAKAMURA Hirotaka  長崎大学, 医歯薬学総合研究科歯学系, 助教 (70346914)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsインフラマソーム / 歯周炎 / 歯石 / リン酸カルシウム結晶 / 国際情報交換、ドイツ
Outline of Final Research Achievements

The aim of this study is to investigate whether the crystals in dental calculus could induce activation of NLRP3 inflammasome to produce IL-1β in macrophages and PMNs. WT macrophage incubated with untreated dental calculus produced a robust amount of IL-1β, whereas the cells incubated with heat-treated calculus produced much less IL-1β. After LPS priming, WT macrophage incubated with heat-treated calculus produced considerable level of IL-1β. However, Nlrp3-/- macrophage produced marginal level of IL-1β. Addition of Z-VAD-FMK significantly suppressed the IL-1β production. Similarly, human PMN incubated with heat-treated calculus produced IL-1β. The IL-1β production by PMN was also inhibited by Z-VAD-FMK, indicating involvement of caspase 1.These results indicate that the crystals in dental calculus could accelerate production of IL-1β through NLRP3 inflammasome activation in mouse macrophage and human PMNs.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (2 results)

All 2012 Other

All Presentation (2 results)

  • [Presentation] The crystals in dental calculus accelerate IL-1β production thorough activation of NLRP3 inflammasome in mouse macrophage and human peripheral polymorphonuclear leukocyte2012

    • Author(s)
      Nakamura H, Yoshimura A, Kaneko T, Latz E, Hara Y
    • Organizer
      12th International Endotoxin and Innate Immunity Society Meeting
    • Place of Presentation
      Tokyo, Japan
    • Related Report
      2012 Research-status Report
  • [Presentation] 歯石中のリン酸カルシウム結晶はNLRP3インフラマソームを活性化する

    • Author(s)
      中村弘隆、吉村篤利、金子高士、Eicke Latz、原宜興
    • Organizer
      2012年度日本歯周病学会九州5大学研修会
    • Place of Presentation
      福岡
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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