A methodological study on infection control for safe clinical application of iPS cells

• Project/Area Number: 24792371
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Social dentistry
• Research Institution: Tsurumi University
• Principal Investigator: OKADA Ayako 鶴見大学, 歯学部, 寄附講座助教 (60515584)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: 機能水 / 口腔再生医療 / 感染制御 / 細胞毒性 / 再生医療 / 次亜塩素酸電解水 / 歯周組織再生医療

Outline of Final Research Achievements

In oral regenerative medicine, such as clinical application of iPS cells, infection control is of the utmost importance in order to maintain the healthy oral environmental balance. In recent years, electrolyzed saline solutions are gaining interests among the medical and dental professionals for their anti-bacterial potentials in shortest possible application time. In the present study, some reactive oxygen species (ROS) based electrolyzed saline solutions containing hypochlorous acid (HClO); also known as super-oxidized water (SOW) have been examined for their anti-bacterial effects, potency of biofilm penetration and cytotoxicity. Results have indicated that SOW containing 125 (~145) mg/L active chlorine and 6.8 pH value can be effective enough to control oral pathogens. In addition, it was understood that this kind of highly purified SOW might be useful in oral regenerative medicine as it showed very low level of cytotoxicity on KB cells derived from pharyngeal mucosal epithelium.

Research Products

• [Journal Article] Effects of Lactobacillus crispatus as a candidate for oral probiotic bacteria on Haemophilus influenzae. (2015)
  • Author(s): Kaoru Sogabe-Ashigaki, Susumu Imai, Ayako Okada, Khairul Matin, Tomohiko Terai, Takekazu Okumura, Nobuhiro Hanada, Hiroshi Kawahara
  • Journal Title: Asian Pacific Journal of Dentistry Volume: 15 Pages: 3-11
  • NAID: 40020570176
  • Peer Reviewed / Open Access
• [Presentation] 新規3DSトレ－を用いた次亜塩素酸電解水の臨床的評価 (2015)
  • Author(s): 岡田彩子, 村田貴俊, Khairul Matin, 宮之原真由, 芦垣 薫, 大塚良子, 須藤智省, 松澤匡純, 桐原 大, 武内博朗, 花田信弘
  • Organizer: 第16回口腔機能水学会学術大会
  • Place of Presentation: 鶴見大学（神奈川県）
  • Year and Date: 2015-03-28 – 2015-03-29
• [Presentation] Cytotoxicity of Reactive Oxygen Species Based Disinfectants on Epithelial Cell (2015)
  • Author(s): A. Okada, K. Matin, S. Imai, T. Kikuchi and N. Hanada
  • Organizer: 93rd General Session & Exhibition of the IADR
  • Place of Presentation: Hynes Convention Center (Boston, USA)
  • Year and Date: 2015-03-11 – 2015-03-14
• [Presentation] Antibacterial Effects of a Flavored Superoxidized Water on Oral Pathogens (2015)
  • Author(s): K. Matin, A. Okada, K. Nebuka, S. Imai, N. Hanada and J. Tagami
  • Organizer: 93rd General Session & Exhibition of the IADR
  • Place of Presentation: Hynes Convention Center (Boston, USA)
  • Year and Date: 2015-03-11 – 2015-03-14
• [Presentation] Probiotic Effects of Lactobacillus crispatus YIT 12319 on Haemophilus influenzae Associated with Chronic Obstructive Pulmonary Disease (2014)
  • Author(s): K. ASHIGAKI, S. IMAI1, G. AKIMARU, A. OKADA, T. OKUMURA, T. TERAI, K. YAMAJI, N. HANADA and H. KAWAHARA
  • Organizer: the 62nd Annual meeting of JADR
  • Place of Presentation: KKRホテル大阪（大阪府）
  • Year and Date: 2014-12-04 – 2014-12-05
• [Presentation] Application of Chimeric Glucanase of Mutanase and Dextranase for the Prevention of Dental Biofilm Formation (2014)
  • Author(s): R. OTSUKA, S. IMAI, T. MURATA, Y. NOMURA, M. OKAMOTO, H. TSUMORI, M. MIYANOHARA, A. OKADA, E. KAKUTA, N. HANADA and Y. MOMOI
  • Organizer: the 62nd Annual meeting of JADR
  • Place of Presentation: KKRホテル大阪（大阪府）
  • Year and Date: 2014-12-04 – 2014-12-05
• [Presentation] ミュータンスレンサ球菌およびLactobacillus gasseriの相互作用について (2014)
  • Author(s): 菊地朋宏, 今井奬, 岡田彩子, 奥村剛一, 花田信弘
  • Organizer: 第63回日本口腔衛生学会・総会
  • Place of Presentation: 熊本市民会館崇城大学ホール（熊本県）
  • Year and Date: 2014-05-29 – 2014-05-31
• [Presentation] 全身的な健康を歯科から考える"予防医科"としての概念を歯科へ 『3DS除菌外来』の試み (第一報) (2014)
  • Author(s): 角田衣理加, 山田秀則, 岡田彩子, 宮之原真由, 阿保備子, 村田貴俊, 武内博朗, 野村義明, 花田信弘
  • Organizer: 第63回日本口腔衛生学会・総会
  • Place of Presentation: 熊本市民会館崇城大学ホール（熊本県）
  • Year and Date: 2014-05-29 – 2014-05-31
• [Presentation] 活性酸素種のKB細胞に対する細胞毒性の検討 (2013)
  • Author(s): 岡田彩子
  • Organizer: 第62回口腔衛生学会・総会
  • Place of Presentation: 長野県松本市キッセイ文化ホ－ル
• [Presentation] 2種次亜塩素酸電解水の細胞毒性に関する検討 (2013)
  • Author(s): 岡田彩子
  • Organizer: 第139回日本歯科保存学会2013年度秋季学術大会
  • Place of Presentation: 秋田県総合生活文化会館
• [Presentation] Streptococcus anginosus用選択培地の開発 (2012)
  • Author(s): 福田雄、西川智子、岡田彩子、村田貴俊、阿保備子、角田衣理加、宮之原真由、野村義明、今井奬、花田信弘
  • Organizer: 第61回口腔衛生学会・総会
  • Place of Presentation: 神奈川歯科大学（神奈川県）
• [Presentation] iPS細胞の安全性に関する検討 第2報 核型異常について (2012)
  • Author(s): 野村義明、石川美佐緒、新井千博、八城祐一、野田晃司、山口貴央、岡田彩子、角田衣理加、山田秀則、今井奬、花田信弘
  • Organizer: 第61回口腔衛生学会・総会
  • Place of Presentation: 神奈川歯科大学（神奈川県）
• [Presentation] Probiotic Effects of Lactobacilli on Cariogenic Biofilm Formation (2012)
  • Author(s): OKADA A, IMAI S, OKUMURA T, TERAI T, NAKAO M, YAMAJI K, MIYANOHARA M, MATIN K, MIYAZAKI K and HANADA N
  • Organizer: The 60th Annual Meeting of Japanese Association for Dental Research
  • Place of Presentation: 新潟コンベンションセンタ－（新潟県）