Development of functional polycation for efficient mRNA delivery system
| Project/Area Number |
24800016
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | The University of Tokyo |
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Principal Investigator |
HIROKUNI Uchida 東京大学, 医学(系)研究科(研究院), 特任研究員 (60637677)
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| Project Period (FY) |
2012-08-31 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | mRNA / ドラッグデリバリー / 機能性高分子 |
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| Research Abstract |
Polyion complexes formed from nucleic acids and polycations, which were termed polypex, has been attracted attention because of their therapeutic potential. Messenger RNA (mRNA) was recognized as a new candidate for the safe nucleic acids drug because of no risk of mutagenesis. However mRNA possessed labile nature in physiological condition. Herein, we synthesized the polycations possessing slightly different chemical structure in their side chain and compared their mRNA delivery mechanism to get the insight for the design of polycations achieving safe and efficient mRNA delivery. In the results, the polycations possessing odd-numbered aminoethylene units in their side chain showed efficient mRNA delivery and higher cytoplasmic stability than those even one. We concluded that the fine-tuning of chemical structure of polycations can enhance the cytoplasmic stability and mRNA expression of polyplexes.
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Report
(3 results)
Research Products
(8 results)
