Exhaled breath analysis for lung cancer detection using ion mobility spectrometry

• Project/Area Number: 24800068
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Tumor diagnosis
• Research Institution: St. Marianna University School of Medicine
• Principal Investigator: HANDA Hiroshi 聖マリアンナ医科大学, 医学部, 助教 (60636265)
• Project Period (FY): 2012-08-31 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
• Keywords: 腫瘍診断学 / 分析科学

Research Abstract

The exhaled breath of 61 patients with lung cancer confirmed by bronchoscopy, were analyzed using ion mobility spectrometry (IMS) and compared with 40 healthy volunteers. As a secondary assessment, we compared adenocarcinoma patients with and without epidermal growth factor receptor (EGFR) mutation. One hundred-five separated volatile organic compound (VOC) peaks were analyzed statistically. Of these, thirteen peaks showed significantly higher levels of lung cancer (p<0.05). n-Dodecane was the most important biomarker to discriminate between lung cancer and healthy subjects with a sensitivity of 90% and a specificity of 69%. Incorporating a decision tree algorithm starting with n-Dodecane peak, a sensitivity of 100 % and specificity of 93 % was achieved. Fifteen adenocarcinoma patients harboring EGFR mutation displayed a significantly higher n-Dodecane peak with a sensitivity of 53 % and a specificity of 100 % (p<0.01) than in 17 adenocarcinoma patients without the EGFR mutation.

Research Products

• [Journal Article] 肺癌の呼気分析による診断 (2012)
  • Author(s): 半田 寛
  • Journal Title: 医学のあゆみ Volume: 240巻 第11号 Pages: 933-935
• [Presentation] Volatile metabolites arising from lung cancer in Japan (2013)
  • Author(s): Handa H, Inoue T, Yonemura Y, Maddula S, Baumbach JI, and Miyazawa T
  • Organizer: Breath Analysis Summit 2013
  • Place of Presentation: Saarbrucken, Germany
• [Presentation] Volatile metabolites arising from lung cancer in Japan (2013)
  • Author(s): Hiroshi Handa
  • Organizer: Breath Summit 2013
  • Place of Presentation: ザールブリュッケン、ドイツ
• [Presentation] Volatile metabolites arising from lung cancer in Japan (2013)
  • Author(s): Hiroshi Handa
  • Organizer: Breath Analysis Summit 2013
  • Place of Presentation: ザールブルッケン、ドイツ
• [Presentation] Detection of lung cancer using ion mobility spectrometry in Japan : pilot study (2012)
  • Author(s): Handa H, Miyazawa T, Morikawa K, Inoue T, Furuya N, Kida H, Nishine H, Nobuyama S, Inoue T, Mineshita M, and Hiramoto T
  • Organizer: ERS annual congress
  • Place of Presentation: Amsterdam, Netherland
• [Presentation] 肺癌におけるイオン易動度分光測定式探知器 (IMS) による呼気分析の検討 (2012)
  • Author(s): 半田寛, 井上哲兵, 森川慶, 古屋直樹, 木田博隆, 西根広樹, 延山誠一, 井上健男, 峯下昌道, 宮澤輝臣
  • Organizer: 第52回日本呼吸器学会学術講演会
  • Place of Presentation: 神戸, 日本
• [Book] 癌・腫瘍学肺癌の呼気分析による診断医学のあゆみ240 (2012)
  • Author(s): 半田寛, 宮澤輝臣
  • Publisher: 医歯薬出版株式会社