The elucidation of molecular mechanism for increase in myoglobin expression and the examination of its optimal expression condition.
Project/Area Number |
24800074
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Research Category |
Grant-in-Aid for Research Activity Start-up
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Allocation Type | Single-year Grants |
Research Field |
Sports science
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Research Institution | Doshisha University |
Principal Investigator |
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Project Period (FY) |
2010 – 2013
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Project Status |
Completed (Fiscal Year 2013)
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Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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Keywords | 持久的トレーニング / ミオグロビン / 転写因子 / 筋組織 / ミトコンドリア / 持久性トレーニング / 骨格筋 / シグナル伝達 |
Research Abstract |
Endurance exercise training (eTR) improves muscle oxidative capacity via an increase in both mitochondrial protein and myoglobin (Mb). Overexpression of peroxisome proliferator-activated receptor γ coactivator-1α (PGC-1α) increases Mb expression in skeletal muscle. Knockout of PGC-1α, however, does not attenuate eTR-induced increase in Mb expression. Therefore, it is controversial how PGC-1α is involved in eTR-induced increase in Mb expression in skeletal muscle. The purpose of this study was to verify whether an increase in PGC-1α expression in skeletal muscle is required for the eTR-induced increase in Mb expression. Timing of increase in Mb expression due to eTR was different from that of mitochondria protein (COX IV). PGC-1α expression did not show any significant differences during the training protocol in this study. Moreover, the PGC-1α mRNA knockdown by siRNA transfection did not attenuate Mb mRNA expression in C2C12 cells. In conclusion, PGC-1α is not essential for the eTR-induced increase in Mb expression in skeletal muscle.
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Report
(3 results)
Research Products
(10 results)
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[Presentation] PGC-1a expression is not essential for endurance training-induced increase in myoglobin expression in skeletal muscle2014
Author(s)
Takakura H, Tanaka G, Tanaka S, Kato H, Shibahara T, Masuda S, Iwanaka N, Hata T, Izawa T, Jue T & Masuda K
Organizer
American College of Sports Medicine 61st Annual Meeting
Place of Presentation
Orland, FL, USA)
Year and Date
2014-05-30
Related Report
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[Presentation] PGC-1α expression is not essential for endurance training-induced increase in myoglobin expression in skeletal muscle.2014
Author(s)
Hisashi Takakura, Goki Tanaka, Seichi Tanaka, Hisashi Kato, Takuya Shibahara, Shinya Masuda, Nobumasa Iwanaka, Toshimichi Hata, Tetsuya Izawa, Thomas Jue and Kazumi Masuda
Organizer
ACSM’s 61st Annual Meeting
Place of Presentation
Orange County Convention Center and the Rosen Centre Hotel Orlando, Florida, USA
Related Report
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