Mechanism-based crosslinkers of acyl carrier protein dehydratase
| Project/Area Number |
24810015
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Living organism molecular science
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Project Period (FY) |
2012-08-31 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 生合成 / 脂肪酸合成酵素 / ポリケタイド合成酵素 / パンテテインアナログ / タンパク質-タンパク質相互作用 / 不可逆的阻害剤 / タンパク質-タンパク質相互作用解析 |
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| Research Abstract |
We reported the design, synthesis, and application of sulfonyl 3-alkynyl pantetheinamides that can cross-link acyl carrier proteins (ACPs) with beta-hydroxy-ACP dehydratase (DH) domains. This cross-linking strategy provides potential to investigate these enzymes in their native binding states by offering easy access to mechanistically cross-linked enzyme species.
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Report
(3 results)
Research Products
(23 results)
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[Journal Article] Trapping the dynamic acyl carrier protein in fatty acid biosynthesis2014
Author(s)
Chi Nguyen, Robert W. Haushalter, D.John Lee, Phineus R.L.Markwick, Joel Bruegger, Grace Caldara-Festin, Kara Finzel, David R.Jackson, Fumihiro Ishikawa, Bing O'Dowd, J.Andrew McCammon, Stanley J.Opella, Shiou-Chuan Tsai, Michael D.Burkart
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Journal Title
Nature
Volume: 505
Issue: 7483
Pages: 427-431
DOI
Related Report
Peer Reviewed
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