| Project/Area Number |
24810019
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Chemical biology
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| Research Institution | Kyushu Institute of Technology |
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Principal Investigator |
YAMANAKA Makoto 九州工業大学, バイオマイクロセンシング技術研究センター, 研究員 (80637455)
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| Project Period (FY) |
2012-08-31 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 生体分子計測 / ヒト膜タンパク質 / リポソーム / バイオセンサ / GPCR / 膜タンパク質 |
|---|
| Research Abstract |
We developed a novel technique to produce cell-derived liposomes which have specific membrane proteins expressed in human cells, and applied those liposomes to a QCM (quartz crystal microbalance) sensor for detecting interactions between membrane proteins and their ligands. Using this technique and gene transfection, we succeeded in producing liposomes which have Bradykinin B2 receptors, GPCRs for bradykinin, and detecting bradykinin using the sensor where the liposomes were immobilized.
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