| Budget Amount *help |
¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Research Abstract |
In this study, we attempted to uncover how the long non-coding RNAs (lncRNAs) regulate transcription via RNA-biding proteins. To investigate the role of arginine-methylation of RNA-biding protein TLS, we first developed a monoclonal antibody against arginine-methylated TLS. We performed RNA-biding immunoprecipitation assays using HeLa lysate and this antibody. We found that the lncRNA transcribed from cyclin D1 promoter binds to methylated TLS by RT-PCR. Our preliminary data also suggest that arginine methylation of TLS might enhance the biding to RNA oligonucleotides. These results suggest that the RNA-dependent transcription is regulated by arginine-methylation of RNA-biding protein.
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