| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Research Abstract |
Amphibians display flexible phenotypes depending on environmental conditions. Larvae of the Hokkaido salamander, Hynobius retardatus, exhibit two distinct morphs, "attack morphs" and "defense morphs", as an inducible phenotypic response to prey and predators, respectively. The presence of their prey, Rana pirica tadpoles, induces a broad-headed attack morph. The presence of predatory dragonfly nymphs (Aeshna nigroflava) induces a defense morph, characterized by enlarged external gills and a high tail. However, the molecular mechanisms underlying these phenotypic changes have yet to be elucidated. To reveal the landscape of molecular mechanisms underlying these inducible plastic morphs, we carried out a de novo transcriptome analysis of Hokkaido salamander larvae. We extracted and sequenced RNAs from the sampled tissues. Then, we compared the obtained reads and contigs of the treatment samples and identified differentially expressed genes.
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