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Structural and functional analysis of a novel iron-sulpfur protein involved in the intracellular assembly of light-harvesting antenna

Research Project

Project/Area Number 24870030
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Functional biochemistry
Research InstitutionKurume National College of Technology

Principal Investigator

HAGIWARA Yoshinori  久留米工業高等専門学校, 生物応用化学科, 助教 (10628548)

Project Period (FY) 2012-08-31 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords鉄硫黄タンパク質 / 光合成 / X線結晶構造解析
Research Abstract

In this study, we focused on the molecular mechanism of the intracellular assembly of supramolecular complex. Especially, we studied the structure and function of the protein, involved in the assembly of cyanobacterial light harvesting antennae complex on the thylakoid membrane. We constructed cyanobacterial Ycf34 expression plasmids and purified those proteins. We found the expressed Ycf34 holds iron-sulfur cluster in the protein. The mutation analysis indicated that each of five cysteine residues of Ycf34 involved in the holding of iron-sulfur cluster in the protein.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Annual Research Report
  • Research Products

    (9 results)

All 2014 2012 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (4 results) Remarks (3 results)

  • [Journal Article] Inactivation of the conserved open reading frame ycf34 of Synechocystis sp2012

    • Author(s)
      Thomas Wallner、Yoshinori Hagiwara、Gabor Bernat、Roman Sobotka、Edward J. Reijerse、Nicole Frankenberg,. Dinkel and Annegret Wilde
    • Journal Title

      PCC 6803 interferes with the photosynthetic electron transport chain、Biochimica et Biophysica Acta–Bioenergetics

      Volume: 11 Issue: 11 Pages: 1817-1817

    • DOI

      10.1016/j.bbabio.2012.06.002

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Inactivation of the conserved open reading frame ycf34 of Synechocystis sp. PCC 6803 interferes with the photosynthetic electron transport chain,2012

    • Author(s)
      Thomas Wallner, Yoshinori Hagiwara, Gabor Bernat, Roman Sobotka, Edward J. Reijerse, Nicole Frankenberg-Dinkel, Annegret Wilde
    • Journal Title

      Biochimica et Biophysica Acta - Bioenergetics

      Volume: 1817 Pages: 2016-2026

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Presentation] 光受容色素の合成に関与するタンパク質の発現および精製2014

    • Author(s)
      土屋順司、碓井美咲、小崎香奈、萩原義徳
    • Organizer
      第19回高専シンポジウムin久留米
    • Place of Presentation
      久留米工業高等専門学校(福岡県)
    • Year and Date
      2014-01-25
    • Related Report
      2013 Final Research Report
  • [Presentation] 光合成集光装置の細胞内構築に関わる新規鉄硫黄タンパク質の発現系の構築と精製2014

    • Author(s)
      萩原義徳、津村優太、平山亜理沙、杉野美里、Nicole Frankenberg-Dinkel
    • Organizer
      第19回高専シンポジウムin久留米
    • Place of Presentation
      久留米工業高等専門学校(福岡県)
    • Year and Date
      2014-01-25
    • Related Report
      2013 Final Research Report
  • [Presentation] 光合成集光装置の細胞内構築に関わる新規鉄硫黄タンパク質の発現系の構築と精製2014

    • Author(s)
      萩原義徳、津村優太、平山亜理沙、杉野美里、Nicole Frankenberg-Dinkel
    • Organizer
      第19回高専シンポジウムin久留米
    • Place of Presentation
      久留米工業高等専門学校
    • Related Report
      2013 Annual Research Report
  • [Presentation] 光受容色素合成に関与するタンパク質の発現及び精製2014

    • Author(s)
      土屋順司、碓井美咲、小崎香奈、萩原義徳
    • Organizer
      第19回高専シンポジウムin久留米
    • Place of Presentation
      久留米工業高等専門学校
    • Related Report
      2013 Annual Research Report
  • [Remarks]

    • URL

      http://apollo.cc.kurume-nct.ac.jp/~hagiwara/

    • Related Report
      2013 Final Research Report
  • [Remarks] 独立行政法人国立高等専門学校機構 久留米工業高等専門学校 生物応用化学科 萩原研究室

    • URL

      http://apollo.cc.kurume-nct.ac.jp/~hagiwara/

    • Related Report
      2013 Annual Research Report
  • [Remarks] 久留米高専 生物応用化学科 萩原研究室

    • URL

      http://www.cc.kurume-nct.ac.jp/BCAC/teacher/lab/hagiwara/index.html

    • Related Report
      2012 Annual Research Report

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Published: 2012-11-27   Modified: 2019-07-29  

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