| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Research Abstract |
This study aimed to create the analysis system to understand how pattern formation and cell behavior are mutually related during early embryogenesis of the spider Parasteatoda tepidariorum. Multiple FISH and 2-step cell labeling methods are now available in this spider system. These methods in combination with embryonic RNA interference make it possible to examine RNAi effects on expression of multiple genes simultaneously and to analyze movement of knockdown-cells compared to surrounding intact cells. Furthermore, when combined with parental RNA interference, RNA sequencing is shown to be a powerful method for searching for target genes of Hedgehog signaling that is possible to regulate both pattern formation and cell behavior in the early spider embryo.
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